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T 세포 백혈병 세포주에서 T 세포 수용체 β쇄 CDR3 유전자 염기서열의 규명

Other Titles
 Identification of the CDR3 Gene Sequence on the β Chain of the T Cell Receptor in T Cell Leukemia Cell Line 
Authors
 최용준  ;  최인홍  ;  김주덕  ;  김세종 
Citation
 Korean Journal of Immunology (대한면역학회지), Vol.19(4) : 541-548, 1997 
Journal Title
 Korean Journal of Immunology (대한면역학회지) 
ISSN
 1015-6453 
Issue Date
1997
Keywords
T cell leukemia ; T cell receptor β chain ; CDR3
Abstract
In order to develop a method for the detection of minimal residual leukemic disease (MRD) in T cell acute lymphocytic leukemia (T cell ALL), T cell leukemia cell line was used to detect clonal TCR p chain mRNA and to synthesize CDR3 specific oligonucleotide probe. For the Jurkat cell line clonal TCR p chain cDNA was amplified by using RT-PCR with oligonucleotide primer, Vp universal primer. As the result of RT-PCR an approximate 300 bp fragment of the TCR chain was obtained, and the partial identification of the TCR p chain gene and the amino acid sequence of the fragment were done by gene cloning and sequencing. The gene sequence of TCR p obtained was identified as Vp8-Jp1.2-Cp2. Diversity gene segment could not be found. Within the p chain, the CDR3 region was identified as 12 amino acids (SFSTCSANYGYT). It is kown that TCR is expressed in about 40% of the all T cell ALL. However it is not kown what percentage of the TCR p chain mRNA expression translates into the actual TCR molecule. It is not certain how many patients with MRD can be detected by this method used in this study, but this technique might be useful to detect MRD in at least 40% of the patients with T-cell ALL
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Se Jong(김세종)
Choi, In Hong(최인홍) ORCID logo https://orcid.org/0000-0001-9851-0137
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/177587
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