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Triton X-100 induces apoptosis in human hepatoma cell lines

Authors
 Ju Mi Ahn  ;  Se Jong Kim  ;  Hoguen Kim  ;  Chanil Park  ;  Won Ho Kim  ;  Jeon Han Park 
Citation
 YONSEI MEDICAL JOURNAL, Vol.38(1) : 52-59, 1997 
Journal Title
 YONSEI MEDICAL JOURNAL 
ISSN
 0513-5796 
Issue Date
1997
MeSH
Apoptosis* ; Carcinoma, Hepatocellular/genetics ; Carcinoma, Hepatocellular/pathology* ; DNA Fragmentation ; Detergents/pharmacology* ; Humans ; Liver Neoplasms/genetics ; Liver Neoplasms/pathology* ; Octoxynol/pharmacology* ; Tumor Cells, Cultured/drug effects
Abstract
The detergent Triton X-100 was used to establish a model for apoptosis in hepatoma cell lines. The electrophoresis of DNA extracted from 0.01% Triton X-100 treated hepatoma cell lines showed DNA ladder formation, a hallmark of apoptosis. The DNA fragmentation appeared within less than 60 min of the Triton X-100 treatment. Chromatin condensation and apoptotic bodies were observed by hematoxylin and eosin (H & E) stain, and fragmented nucleosome was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling (TUNEL) test. Apoptosis was semi-quantitated by measuring the lactate dehydrogenase (LDH) level for cytotoxity. It was found that apoptosis had been induced in more than 90% of the cells treated with Triton X-100 for 150 min. These data show that Triton X-100 efficiently induces the apoptotic cell death in hepatoma cell lines.
Files in This Item:
T199701217.pdf Download
DOI
10.3349/ymj.1997.38.1.52
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Se Jong(김세종)
Kim, Won Ho(김원호) ORCID logo https://orcid.org/0000-0002-5682-9972
Kim, Hogeun(김호근)
Park, Jeon Han(박전한) ORCID logo https://orcid.org/0000-0001-9604-3205
Park, Chan Il(박찬일)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/177424
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