Cited 10 times in
Triton X-100 induces apoptosis in human hepatoma cell lines
DC Field | Value | Language |
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dc.contributor.author | 김세종 | - |
dc.contributor.author | 김원호 | - |
dc.contributor.author | 김호근 | - |
dc.contributor.author | 박전한 | - |
dc.contributor.author | 박찬일 | - |
dc.date.accessioned | 2020-07-03T17:18:15Z | - |
dc.date.available | 2020-07-03T17:18:15Z | - |
dc.date.issued | 1997 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/177424 | - |
dc.description.abstract | The detergent Triton X-100 was used to establish a model for apoptosis in hepatoma cell lines. The electrophoresis of DNA extracted from 0.01% Triton X-100 treated hepatoma cell lines showed DNA ladder formation, a hallmark of apoptosis. The DNA fragmentation appeared within less than 60 min of the Triton X-100 treatment. Chromatin condensation and apoptotic bodies were observed by hematoxylin and eosin (H & E) stain, and fragmented nucleosome was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling (TUNEL) test. Apoptosis was semi-quantitated by measuring the lactate dehydrogenase (LDH) level for cytotoxity. It was found that apoptosis had been induced in more than 90% of the cells treated with Triton X-100 for 150 min. These data show that Triton X-100 efficiently induces the apoptotic cell death in hepatoma cell lines. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Yonsei University | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Apoptosis* | - |
dc.subject.MESH | Carcinoma, Hepatocellular/genetics | - |
dc.subject.MESH | Carcinoma, Hepatocellular/pathology* | - |
dc.subject.MESH | DNA Fragmentation | - |
dc.subject.MESH | Detergents/pharmacology* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Liver Neoplasms/genetics | - |
dc.subject.MESH | Liver Neoplasms/pathology* | - |
dc.subject.MESH | Octoxynol/pharmacology* | - |
dc.subject.MESH | Tumor Cells, Cultured/drug effects | - |
dc.title | Triton X-100 induces apoptosis in human hepatoma cell lines | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학교실) | - |
dc.contributor.googleauthor | Ju Mi Ahn | - |
dc.contributor.googleauthor | Se Jong Kim | - |
dc.contributor.googleauthor | Hoguen Kim | - |
dc.contributor.googleauthor | Chanil Park | - |
dc.contributor.googleauthor | Won Ho Kim | - |
dc.contributor.googleauthor | Jeon Han Park | - |
dc.identifier.doi | 10.3349/ymj.1997.38.1.52 | - |
dc.contributor.localId | A00603 | - |
dc.contributor.localId | A00774 | - |
dc.contributor.localId | A01183 | - |
dc.contributor.localId | A01641 | - |
dc.contributor.localId | A01710 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 9100483 | - |
dc.contributor.alternativeName | Kim, Se Jong | - |
dc.contributor.affiliatedAuthor | 김세종 | - |
dc.contributor.affiliatedAuthor | 김원호 | - |
dc.contributor.affiliatedAuthor | 김호근 | - |
dc.contributor.affiliatedAuthor | 박전한 | - |
dc.contributor.affiliatedAuthor | 박찬일 | - |
dc.citation.volume | 38 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 52 | - |
dc.citation.endPage | 59 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.38(1) : 52-59, 1997 | - |
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