Computer-Assisted Instruction(CAI) is one kind of teaching method to teach leaners knowledge, attitude, and technical aspect of course content through utilizing a computer as a learning material. CAI is focused on the concept of interaction and individualized leaning. Thus in teaching and learning process CAI is considered the individual differences of leaners. And learners can study course contents repeatedly through utilizing courseware. Based on the importance of individualized learning program through utilizing a computer the courseware of infection control was developed to acquire and apply the appropriate knowledge for controlling infection for learners.