0 94

Cited 0 times in

Transmission and regulation of biochemical stimulus via a nanoshell directly adsorbed on the cell membrane to enhance chondrogenic differentiation of mesenchymal stem cell

Authors
 Uiyoung Han  ;  Jun‐Ha Hwang  ;  Jong‐Min Lee  ;  Hyeoni Kim  ;  Han‐Sung Jung  ;  Jeong‐Ho Hong  ;  Jinkee Hong 
Citation
 BIOTECHNOLOGY AND BIOENGINEERING, Vol.117(1) : 184-193, 2020 
Journal Title
 BIOTECHNOLOGY AND BIOENGINEERING 
ISSN
 0006-3592 
Issue Date
2020
Keywords
chondrogenic differentiation ; extracellular matrix ; mesenchymal stem cell ; polymeric nanofilm
Abstract
A nanoscale artificial extracellular matrix (nanoshell) formed by layer-by-layer adsorption can enhance and modulate the function of stem cells by transferring biochemical stimulus to the cell directly. Here, the nanoshell composed of fibronectin (FN) and chondroitin sulfate (CS) is demonstrated to promote chondrogenic differentiation of mesenchymal stem cells (MSCs). The multilayer structure of nanoshell is formed by repeating self-assembly of FN and CS, and its thickness can be controlled through the number of layers. The expression of chondrogenic markers in MSCs coated with the FN/CS nanoshell was increased as the number of bilayers in the nanoshell increased until four, but when it exceeds five bilayers, the effect began to decrease. Finally, the MSCs coated with optimized four bilayers of FN/CS nanoshell have high chondrogenic differentiation efficiency and showed the potential to increase formation of cartilage tissue when it is transplanted into mouse kidney. So, the precise regulation of stem cell fate at single cell level can be possible through the cellular surface modification by self-assembled polymeric film.
Full Text
https://onlinelibrary.wiley.com/doi/full/10.1002/bit.27183
DOI
10.1002/bit.27183
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Lee, Jong Min(이종민) ORCID logo https://orcid.org/0000-0002-9466-7644
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/174916
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links