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Cis-control of Six1 expression in neural crest cells during craniofacial development

Authors
 Zhaoming Wu  ;  Yanxia Rao  ;  Sushan Zhang  ;  Eun‐Jung Kim  ;  Shinya Oki  ;  Hidemitsu Harada  ;  Martin Cheung  ;  Han‐Sung Jung 
Citation
 DEVELOPMENTAL DYNAMICS, Vol.248(12) : 1264-1272, 2019 
Journal Title
 DEVELOPMENTAL DYNAMICS 
ISSN
 1058-8388 
Issue Date
2019
Keywords
Six1 ; craniofacial development ; enhancer ; neural crest
Abstract
BACKGROUND: Six1 is a transcriptional factor that plays an important role in embryonic development. Mouse and chick embryos deficient for Six1 have multiple craniofacial anomalies in the facial bones and cartilages. Multiple Six1 enhancers have been identified, but none of them has been reported to be active in the maxillary and mandibular process. RESULTS: We studied two Six1 enhancers in the chick neural crest tissues during craniofacial development. We showed that two evolutionarily conserved enhancers, Six1E1 and Six1E2, act synergistically. Neither Six1E1 nor Six1E2 alone can drive enhancer reporter signal in the maxillary or mandibular processes. However, their combination, Six1E, showed robust enhancer activity in these tissues. Similar reporter signal can also be driven by the mouse homolog of Six1E. Mutations of multiple conserved transcriptional factor binding sites altered the enhancer activity of Six1E, especially mutation of the LIM homeobox binding site, dramatically reduced the enhancer activity, implying that the Lhx protein family be an important regulator of Six1 expression. CONCLUSION: This study, for the first time, described the synergistic activation of two Six1 enhancers in the maxillary and mandibular processes and will facilitate more detailed studies of the regulation of Six1 in craniofacial development.
Full Text
https://anatomypubs.onlinelibrary.wiley.com/doi/full/10.1002/dvdy.109
DOI
10.1002/dvdy.109
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eun-Jung(김은정) ORCID logo https://orcid.org/0000-0002-9515-7590
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/174715
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