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The prediction of interferon-α therapeutic effect by sequence variation of the HCV hypervariable region 1

Authors
 Byung Il Yeh  ;  Hyun Won Kim  ;  Hyon Suk Kim  ;  Jong Young Lee  ;  Kwang Ho Lee  ;  Kang Mi Lee  ;  Jin Suk Kim  ;  Kwang Hyub Han 
Citation
 Yonsei Medical Journal, Vol.40(5) : 430-438, 1999 
Journal Title
YONSEI MEDICAL JOURNAL
ISSN
 0513-5796 
Issue Date
1999
MeSH
Adult ; Aged ; Amino Acid Sequence ; Female ; Genotype ; Hepacivirus/classification* ; Hepatitis C/drug therapy* ; Hepatitis C/virology ; Humans ; Interferon-alpha/therapeutic use* ; Male ; Middle Aged ; Molecular Sequence Data ; Viral Nonstructural Proteins/blood ; Viral Nonstructural Proteins/chemistry* ; Viral Nonstructural Proteins/genetics
Abstract
Interferon-alpha (IFN-alpha) has been used to treat hepatitis C virus (HCV)-induced hepatitis, but it has been effective in only about half of the treated patients, with recurrence appearing in the other half. As a consequence of the possible complications associated with IFN-alpha and the high cost of treatment, it has become extremely important to select the proper patients for IFN-alpha treatment. In our previous study, we found that the quasispecies in the hypervariable region (HVR) 1 of HCV were various and that a new quasispecies can appear in non-responders and/or lead to deterioration in the patients' condition. The preliminary data we obtained in the process of our previous research led us to believe that the quasispecies of HVR 1 has something to do with the effect of IFN-alpha. Thus, in this investigation, we tried to determine the predictive factors of IFN-alpha therapy. Thirty patients with HCV infection were treated with IFN-alpha. Among them, 15 patients recovered after six months IFN-alpha treatment, but the remaining 15 patients showed no response after six months IFN-alpha treatment. We cloned HVR 1 DNA by reverse transcription-polymerase chain reaction (RT-PCR) and examined the quasispecies of HVR 1. As the quasispecies of HVR 1 in non-responders varied more than in the complete remission group, we concluded that the sequence variation in HVR 1 of HCV can be used to predict the effect of IFN-alpha.
Files in This Item:
T199902197.pdf Download
DOI
10.3349/ymj.1999.40.5.430
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/174126
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