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Identification and purification of IgE-reactive proteins in German cockroach extract

Authors
 Tai Soon Yong  ;  Jong Seok Lee  ;  Jongweon Lee  ;  Soon Jung Park  ;  Soung Hoo Jeon  ;  Han Il Ree  ;  Jung Won Park 
Citation
 Yonsei Medical Journal, Vol.40(3) : 283-289, 1999 
Journal Title
 Yonsei Medical Journal 
ISSN
 0513-5796 
Issue Date
1999
MeSH
Amino Acid Sequence/genetics ; Animals ; Cockroaches/chemistry* ; Humans ; Immunoglobulin E/immunology* ; Insect Proteins/genetics ; Insect Proteins/immunology* ; Insect Proteins/isolation & purification* ; Mice ; Mice, Inbred BALB C ; Tissue Extracts/chemistry*
Abstract
Cockroaches have been implicated as a cause of respiratory allergy in urban areas worldwide. IgE-reactive German cockroach proteins were identified with molecular weights (MWs) of 90, 66, 50, 43 and 36 KD by immunoblot analysis in both immune BALB/c mice and sensitized humans. Prominent IgE-reactive proteins were purified using FPLC by ion-exchange chromatography, gel filtration and hydrophobic chromatography. The N-terminal amino acid sequence of a purified protein with a MW of 66 KD on SDS-PAGE was Val-Thr-Leu-Lys-Lys(Val)-Met-Ile-Lys-Thr-Phe-Tyr. No homologous protein was found through a search of GenBank that indicated a novel IgE-reactive protein in German cockroach extract. Another purified protein with a MW of 36 KD reacted strongly with a monoclonal antibody against Bla g 2.
Files in This Item:
T199902668.pdf Download
DOI
10.3349/ymj.1999.40.3.283
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/173882
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