Cited 3 times in
Identification and purification of IgE-reactive proteins in German cockroach extract
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박순정 | - |
dc.contributor.author | 용태순 | - |
dc.date.accessioned | 2020-01-10T02:06:58Z | - |
dc.date.available | 2020-01-10T02:06:58Z | - |
dc.date.issued | 1999 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/173882 | - |
dc.description.abstract | Cockroaches have been implicated as a cause of respiratory allergy in urban areas worldwide. IgE-reactive German cockroach proteins were identified with molecular weights (MWs) of 90, 66, 50, 43 and 36 KD by immunoblot analysis in both immune BALB/c mice and sensitized humans. Prominent IgE-reactive proteins were purified using FPLC by ion-exchange chromatography, gel filtration and hydrophobic chromatography. The N-terminal amino acid sequence of a purified protein with a MW of 66 KD on SDS-PAGE was Val-Thr-Leu-Lys-Lys(Val)-Met-Ile-Lys-Thr-Phe-Tyr. No homologous protein was found through a search of GenBank that indicated a novel IgE-reactive protein in German cockroach extract. Another purified protein with a MW of 36 KD reacted strongly with a monoclonal antibody against Bla g 2. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Yonsei University | - |
dc.relation.isPartOf | Yonsei Medical Journal | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Amino Acid Sequence/genetics | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cockroaches/chemistry* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunoglobulin E/immunology* | - |
dc.subject.MESH | Insect Proteins/genetics | - |
dc.subject.MESH | Insect Proteins/immunology* | - |
dc.subject.MESH | Insect Proteins/isolation & purification* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred BALB C | - |
dc.subject.MESH | Tissue Extracts/chemistry* | - |
dc.title | Identification and purification of IgE-reactive proteins in German cockroach extract | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Tropica Medicine (열대의학교실) | - |
dc.contributor.googleauthor | Tai Soon Yong | - |
dc.contributor.googleauthor | Jong Seok Lee | - |
dc.contributor.googleauthor | Jongweon Lee | - |
dc.contributor.googleauthor | Soon Jung Park | - |
dc.contributor.googleauthor | Soung Hoo Jeon | - |
dc.contributor.googleauthor | Han Il Ree | - |
dc.contributor.googleauthor | Jung Won Park | - |
dc.identifier.doi | 10.3349/ymj.1999.40.3.283 | - |
dc.contributor.localId | A01545 | - |
dc.contributor.localId | A02424 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 10412341 | - |
dc.contributor.alternativeName | Park, Soon Jung | - |
dc.contributor.affiliatedAuthor | 박순정 | - |
dc.contributor.affiliatedAuthor | 용태순 | - |
dc.citation.volume | 40 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 283 | - |
dc.citation.endPage | 289 | - |
dc.identifier.bibliographicCitation | Yonsei Medical Journal, Vol.40(3) : 283-289, 1999 | - |
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