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Expression of fas ligand in human hepatoma cell lines: role of hepatitis B virus X (HBX) in induction of fas ligand

Authors
 Eui-Cheol Shin  ;  Jeon‐Soo Shin  ;  Jeon‐Han Park  ;  Hoguen Kim  ;  Se‐Jong Kim 
Citation
 International Journal of Cancer, Vol.82(4) : 587-591, 1999 
Journal Title
 International Journal of Cancer 
ISSN
 0020-7136 
Issue Date
1999
MeSH
Apoptosis/physiology* ; Carcinoma, Hepatocellular/metabolism* ; Carcinoma, Hepatocellular/virology ; Fas Ligand Protein ; Hepatitis B virus/genetics ; Humans ; Liver Neoplasms/metabolism* ; Liver Neoplasms/virology ; Membrane Glycoproteins/metabolism ; Membrane Glycoproteins/physiology* ; Trans-Activators/genetics ; Trans-Activators/physiology* ; Transfection ; Tumor Cells, Cultured ; Tumor Escape
Abstract
It has been postulated that tumor cells expressing Fas ligand (FasL) can evade immune surveillance by inducing apoptosis in T cells expressing Fas. In this study, we investigated FasL expression in 13 human hepatoma cell lines. Strong FasL expression was detected by reverse transcription-polymerase chain reaction or immunofluorescence in Hep G2.2.15, in which the hepatitis-B-virus (HBV) genome was transfected, and in SNU-354, which showed HBx transcripts. To determine the biological activity of FasL, Hep G2.2. 15 was co-cultured with MOLT-4, T-cell-leukemia cells. Hep G2.2.15 induced apoptosis in MOLT-4 and this was inhibited by the antagonistic anti-Fas antibody, ZB4. For further analysis of the role of HBx in the induction of FasL, PLC/PRF/5 cells were transfected transiently with the HBV genome, or HBx, or the frameshift mutant of HBx. In PLC/PRF/5 cells transfected with the HBV genome or HBx but not in cells transfected with the frameshift mutant of HBx, FasL expression was detected. Our data suggest that HBx plays a role in the induction of FasL in hepatoma cells and in the escape from immune surveillance.
Files in This Item:
T199901168.pdf Download
DOI
10.1002/(sici)1097-0215(19990812)82:4<587::aid-ijc19>3.0.co;2-9
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Se Jong(김세종)
Kim, Hogeun(김호근)
Park, Jeon Han(박전한) ORCID logo https://orcid.org/0000-0001-9604-3205
Shin, Jeon Soo(신전수) ORCID logo https://orcid.org/0000-0002-8294-3234
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/173848
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