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Two-photon intravital imaging of leukocyte migration during inflammation in the respiratory system

Authors
 Young Min Kim  ;  Soi Jeong  ;  Young Ho Choe  ;  Young-Min Hyun 
Citation
 ACUTE AND CRITICAL CARE, Vol.34(2) : 101-107, 2019 
Journal Title
 ACUTE AND CRITICAL CARE 
ISSN
 2586-6052 
Issue Date
2019
Keywords
inflammation ; intravital imaging ; respiratory system ; two-photon microscopy
Abstract
Two-photon intravital imaging is a powerful method by which researchers are able to directly observe biological phenomena in live organisms. Researchers in various biomedical research fields have applied two-photon imaging to a variety of target organs by utilizing this technology's ability to penetrate to significant depths with minimal phototoxicity. The mouse respiratory system in inflammation models is a good example, as two-photon intravital imaging can provide insights as to how the immune system is activated in response to inflammation within the respiratory system. Inflammation models can be generated via influenza viral, bacterial, or lipopolysaccharide injection. To exteriorize the lungs or trachea, thoracotomy or tracheotomy is performed, respectively; the appropriate combination of inflammation induction and organ exposure is selected depending on the study purpose. On the other hand, visualizing the movement of leukocytes is also an important component; to this end, immune cell populations of interest are either labeled via the genetic attachment of fluorescent proteins or stained with antibodies or dyes. With the proper selection of methods at each step, twophoton intravital imaging can yield visual evidence regarding immune responses to inflammation.
Files in This Item:
T201904757.pdf Download
DOI
10.4266/acc.2019.00542
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Hyun, Young-Min(현영민) ORCID logo https://orcid.org/0000-0002-0567-2039
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/173503
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