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Fas ligand and Fas are expressed constitutively in human astrocytes and the expression increases with IL-1, IL-6, TNF-alpha, or IFN-gamma

 Chulhee Choi  ;  Joo Young Park  ;  Jeonggi Lee  ;  Jung-Hee Lim  ;  Eui-Cheol Shin  ;  Young Soo Ahn  ;  Chul-Hoon Kim  ;  Se-Jong Kim  ;  Joo-Deuk Kim  ;  Il Saing Choi  ;  In-Hong Choi 
 JOURNAL OF IMMUNOLOGY, Vol.162(4) : 1889-1895, 1999 
Journal Title
Issue Date
Adult ; Apoptosis/immunology ; Apoptosis Regulatory Proteins ; Astrocytes/metabolism* ; Cell Membrane/immunology ; Cells, Cultured ; Cytokines/physiology* ; DNA Fragmentation/immunology ; Fas Ligand Protein ; Female ; Fetus ; Humans ; Immunoglobulin M/pharmacology ; Interferon-gamma/physiology ; Interleukin-1/physiology ; Interleukin-6/physiology ; Ligands ; Male ; Membrane Glycoproteins/biosynthesis* ; Membrane Glycoproteins/immunology ; TNF-Related Apoptosis-Inducing Ligand ; Tumor Cells, Cultured ; Tumor Necrosis Factor-alpha/biosynthesis ; Tumor Necrosis Factor-alpha/physiology ; Up-Regulation/immunology ; fas Receptor/biosynthesis* ; fas Receptor/immunology
Fas ligand (FasL) and Fas are mediators of apoptosis, which are implicated in the peripheral deletion of autoimmune cells, activation-induced T cell death, and cytotoxicity mediated by CD8+ T cells. Fas is also believed to be involved in several central nervous system diseases, but until now, the effector cells expressing FasL in the brain have not been identified. We investigated the expression levels of Fas and FasL with the stimulation of cytokines and the possible effector cells targeting Fas-bearing cells. Our data demonstrated that: 1) FasL is expressed constitutively on astrocytes taken from a fetus or an adult and that its expression increases when these cells are treated with IL-1, IL-6, or TNF-alpha in which the pretreatment of IFN-gamma triggers astrocytes to express more FasL; 2) astrocytes induce apoptosis in MOLT-4 cells through FasL; 3) Fas is also expressed constitutively and is up-regulated by IL-1, IL-6, or TNF-alpha in which the pretreatment of IFN-gamma triggers astrocytes to express more Fas; 4) apoptosis occurs when fetal astrocytes are treated with agonistic anti-Fas IgM Ab after culture with IFN-gamma and TNF-alpha; and 5) TNF-related apoptosis inducing ligand is up-regulated in fetal astrocytes with stimuli of IL-1 or TNF-alpha. These findings suggest a possible role of astrocytes in the induction of apoptosis in central nervous system diseases.
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1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Se Jong(김세종)
Choi, In Hong(최인홍) ORCID logo https://orcid.org/0000-0001-9851-0137
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