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Multiple functional P2X and P2Y receptors in the luminal and basolateral membranes of pancreatic duct cells

Authors
 Xiang Luo  ;  Weizhong Zheng  ;  Ming Yan  ;  Min Goo Lee  ;  Shmuel Muallem 
Citation
 AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, Vol.277(2) : C205-C215, 1999 
Journal Title
 AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY 
ISSN
 0363-6143 
Issue Date
1999
MeSH
Animals ; Base Sequence/genetics ; Calcium/metabolism ; Chloride Channels/physiology ; Electric Conductivity ; Female ; GTP-Binding Proteins/metabolism ; Intracellular Membranes/metabolism* ; Male ; Molecular Sequence Data ; Osmolar Concentration ; Pancreatic Ducts/cytology ; Pancreatic Ducts/metabolism* ; Protein Isoforms/agonists ; Protein Isoforms/genetics ; Protein Isoforms/metabolism ; Purinergic P2 Receptor Agonists ; Rats ; Rats, Sprague-Dawley ; Receptors, Purinergic P2/genetics ; Receptors, Purinergic P2/metabolism*
Abstract
Purinergic receptors in the basolateral and luminal membranes of the pancreatic duct can act by a feedback mechanism to coordinate transport activity in the two membranes during ductal secretion. The goal of the present work was to identify and localize the functional P2 receptors (P2R) in the rat pancreatic duct. The lack of selective agonists and/or antagonists for any of the cloned P2R dictated the use of molecular and functional approaches to the characterization of ductal P2R. For the molecular studies, RNA was prepared from microdissected pancreatic intralobular ducts and was shown to be free of mRNA for amylase and endothelial nitric oxide synthase (markers for acinar and endothelial cells, respectively). A new procedure is described to obtain an enriched preparation of single duct cells suitable for electrophysiological studies. Localization of P2R was achieved by testing the effect of various P2R agonists on intracellular Ca(2+) concentration ([Ca(2+)](i)) of microperfused intralobular ducts. RT-PCR analysis suggested the expression of six subtypes of P2R in the pancreatic duct: three P2YR and three P2XR. Activation of Cl(-) current by various nucleotides and coupling of the receptors activated by these nucleotides to G proteins confirmed the expression of multiple P2R in duct cells. Measurement of [Ca(2+)](i) in microperfused intralobular ducts suggested the expression of P2X(1)R, P2X(4)R, probably P2X(7)R, and as yet unidentified P2YR, possibly P2Y(1)R, in the basolateral membrane. Expression of P2Y(2)R, P2Y(4)R, and P2X(7)R was found in the luminal membrane. The unprecedented expression of such a variety of P2R in one cell type, many capable of activating Cl(-) channels, suggests that these receptors may have an important role in pancreatic duct cell function.
Full Text
https://www.physiology.org/doi/full/10.1152/ajpcell.1999.277.2.C205#
DOI
10.1152/ajpcell.1999.277.2.C205
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Lee, Min Goo(이민구) ORCID logo https://orcid.org/0000-0001-7436-012X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/172877
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