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Viability of cells in cryopreserved canine cardiovascular organs for transplantation

 Jong-Chul Park  ;  Hak-Joon Sung  ;  Dong Hee Lee  ;  Young Hwan Park  ;  Bum Koo Cho  ;  Hwal Suh 
 Yonsei Medical Journal, Vol.41(5) : 556-562, 2000 
Journal Title
Issue Date
Animals ; Arteries/transplantation ; Cell Survival ; Coronary Vessels*/transplantation ; Cryopreservation* ; Dogs ; Endothelium, Vascular/cytology ; Endothelium, Vascular/physiology* ; Female ; Male ; Trachea*/transplantation ; Veins/transplantation
Viability, Endothelial Cells, Vessels, Trachea, Cryopreservation
To determine applicability of the cryopreservation procedure for vessel grafts, the viability of endothelial cells (ECs) among the whole cells in three kinds of organs artery, vein, trachea in mongrel dogs was evaluated on the basis of histological analysis. The Griffonia simplicifolia agglutins-fluorescein isothiocyanate (GSA-FITC) and propidium iodide (PI) double staining methods were combined with flow cytometry (FCM), which was able to simultaneously determine the viability of whole cells and ECs from the same tissue, were performed after harvesting, after antibiotic solution treatment, and after cryopreservation and thawing. In most cases, the viability of ECs is lower than that of whole cells from veins and arteries. The viability of whole cells in veins was maintained until the antibiotic solution treatment and then decreased significantly after cryopreservation and thawing, while the ECs began to decrease significantly after the antibiotic solution treatment and more markedly decreased after thawing. The viability of ECs and whole cells from arteries was similar to that of the veins' conditions. The viability of whole cells from the trachea decreased with a similar pattern to that of the ECs from vessels. In consideration of maintaining cell viability among the three kinds of organs, the viability of arteries was better than that of the others. The cells in the trachea demonstrated a lower viability than the vessels. The effect of antibiotic solution treatment on the reduction of cell viability depends on the treatment time and temperature.
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1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
Yonsei Authors
Park, Young Hwan(박영환) ORCID logo https://orcid.org/0000-0001-9802-8017
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
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