0 65

Cited 0 times in

Type I atelocollagen grafting onto ozone induced polyurethane films: cell attachment, proliferation and collagen synthesis

 Jong‐Chul Park  ;  Yu‐Shik Hwang  ;  Jong‐Eun Lee  ;  Ki Dong Park  ;  Kazuaki Matsumura  ;  Suong‐Hyu Hyon  ;  Hwal Suh 
 Journal of Biomedical Materials Research, Vol.52(4) : 669-677, 2000 
Journal Title
 Journal of Biomedical Materials Research 
Issue Date
Animals ; Cell Adhesion ; Cell Differentiation ; Cell Division ; Cells, Cultured ; Coated Materials, Biocompatible/chemistry* ; Collagen/biosynthesis ; Collagen/chemistry* ; Elasticity ; Esophagus/cytology ; Fibroblasts/cytology ; Materials Testing ; Membranes, Artificial* ; Oxidation-Reduction ; Ozone* ; Peroxides/chemical synthesis* ; Polyurethanes/chemistry* ; Rabbits ; Spectrum Analysis ; Surface Properties ; Wettability ; X-Rays
Animals ; Cell Adhesion ; Cell Differentiation ; Cell Division ; Cells ; Cultured ; Coated Materials ; Biocompatible ; chemistry ; Collagen ; biosynthesis ; Elasticity ; Esophagus ; cytology ; Fibroblasts ; Materials Testing ; Membranes ; Artificial ; Oxidation-Reduction ; Ozone ; Peroxides ; chemical synthesis ; Polyurethanes ; Rabbits ; Spectrum Analysis ; Surface Properties ; Wettability ; X-Rays
An approach is presented for the graft copolymerization of type I atelocollagen onto the surface of polyurethane (PU) films treated with ozone. Through inducing oxidization to modify PU surface by ozone, peroxide groups are easily generated on the surface. Those peroxides are broken by redox‐polymerization, and provide active species which initiate graft polymerization by reacting with amines in the collagen molecules. The ozone oxidation time and voltage could readily control the amount of peroxide production. The surface density of generated peroxides on PU surface was determined by iodide method. The maximum concentration of peroxide was about 10.20 × 10−8mol/cm2 when ozone oxidation was performed at 60 V for 30 min. After the reaction of PU by ozone oxidation, type I atelocollagen was graft‐copolymerized onto the PU film. All the physical measurements on the collagen‐grafted surface indicated that the PU surface was effectively covered with type I atelocollagen. The interaction of the collagen‐grafted PU surface with fibroblasts could be greatly enhanced by the surface graft polymerization with type I atelocollagen. Attachment and proliferation of fibroblasts on the grafted type I atelocollagen were significantly enhanced, and it is assumed that the atelocollagen matrix supported the initial attachment and growth of cells. In the early stage of proliferation, collagen synthesis in fibroblasts was not activated and remained at a relatively low level due to the grafted type I atelocollagen, increasing only with fibroblast differentiation. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res, 52, 669–677, 2000.
Full Text
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Lee, Jong Eun(이종은) ORCID logo https://orcid.org/0000-0001-6203-7413
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.