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Using autofluorescence to detect bacterial contamination in root fractures

Authors
 Hye-min Ku  ;  Young Ryul Oh  ;  Eun-Song Lee  ;  Euiseong Kim  ;  Baek-Il Kim 
Citation
 Journal of Dentistry, Vol.86 : 27-32, 2019 
Journal Title
 Journal of Dentistry 
ISSN
 0300-5712 
Issue Date
2019
Keywords
Bacteria ; Biofilms ; Cracked tooth ; Depth ; Fluorescence ; Root fracture
Abstract
OBJECTIVES: Conventional methods for detecting root fractures cannot assess their depth or bacterial contamination. This study was designed to measure the autofluorescence emitted from a root fracture, with the aim of determining whether this is a suitable method for quantifying the depth and bacterial invasion of a fracture. METHODS: This in vitro study investigated 33 mandibular second molars with periapical lesions that had been extracted after finding root fractures in endodontically treated teeth during intentional replantation or diagnostic surgery. The root fractures were scanned using a fluorescence technique, and the association between fluorescence parameters and fracture depth was analyzed. The significance of the association between the red fluorescence among autofluorescence parameters and bacterial contamination within the fracture was examined. RESULTS: When the depth of the root fractures was evaluated by micro computed tomography, the scattering of light in the fractures increased with depth, and there was a gradual increase in the quantitative fluorescence parameter indicating the deepest point (ΔFmax) in the fractures. In addition, we observed red fluorescence on the outer surface of deeper fractures. The tooth fractures exhibiting red fluorescence were evaluated for bacterial contamination associated with red-fluorescent porphyrin, which revealed bacterial invasion into these fractures. On the other hand, non-red-fluorescing fractures contained necrotic tissue, debris, and irritants. CONCLUSIONS: This viable fluorescent technique can potentially quantify the depth of root fractures and be used as a risk indicator for root fractures with periodontal inflammation. CLINICAL SIGNIFICANCE: The auto-fluorescence technique can be used to detect depth and bacterial contamination of root fractures. It is postulated that the auto-fluorescence can be used as a risk indicator of deep fractures and can replace conventional fracture detection methods.
Full Text
https://www.sciencedirect.com/science/article/pii/S0300571219301071
DOI
10.1016/j.jdent.2019.05.024
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Preventive Dentistry and Public Oral Health (예방치과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Baek Il(김백일) ORCID logo https://orcid.org/0000-0001-8234-2327
Kim, Eui Seong(김의성) ORCID logo https://orcid.org/0000-0003-2126-4761
Lee, Eun Song(이은송) ORCID logo https://orcid.org/0000-0002-2949-4783
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/171137
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