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Bcl-2 is a prognostic marker and its silencing inhibits recurrence in ameloblastomas

 Jue Young Kim  ;  Jinsun Kim  ;  Shadavlonjid Bazarsad  ;  In‐Ho Cha  ;  Sung‐Won Cho  ;  Jin Kim 
 ORAL DISEASES, Vol.25(4) : 1158-1168, 2019 
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Issue Date
Bcl-2 ; OPG ; ameloblastoma ; apoptosis ; recurrence
OBJECTIVES: Ameloblastomas are the most common odontogenic epithelial tumors with high recurrence rate. The aim of this study was to identify apoptosis-related genes with recurrence of ameloblastomas and to evaluate its feasibility as a prognostic marker and as a target molecule preventing from recurrence. MATERIALS AND METHODS: Public microarray data were analyzed. To evaluate their expression in ameloblastoma patients, immunohistochemical staining was performed in 89 human ameloblastoma tissues. Quantitative PCR was performed by use of ameloblastoma cell line (AM-1). Fluorescence activated cell sorting analysis and western blotting were conducted following transfection with siRNA. Further, AM-1 cells were implanted in the renal subcapsular layer of immunodeficient mice. RESULTS: Microarray data analysis revealed that osteoprotegerin (OPG) and B-cell lymphoma 2 (Bcl-2) were the two most upregulated genes in ameloblastoma. Only Bcl-2 expression was significantly (p = 0.020) associated with recurrence in conservative treatment group (n = 17) among 89 patients. Silencing of Bcl-2 increased apoptosis in AM-1 cells in vitro and inhibited tumor nodule formation of AM-1 cells in vivo. CONCLUSION: These results suggest that Bcl-2 expression is a useful biomarker to predict recurrence of ameloblastomas, and as a therapeutic target molecule to prevent recurrence of ameloblastoma.
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2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral and Maxillofacial Surgery (구강악안면외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ju Young(김주영)
Kim, Jin(김진)
Kim, Jin Sun(김진선) ORCID logo https://orcid.org/0000-0002-5089-3711
Cho, Sung Won(조성원) ORCID logo https://orcid.org/0000-0001-7505-9769
Cha, In Ho(차인호) ORCID logo https://orcid.org/0000-0001-8259-2190
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