35 54

Cited 12 times in

3D Bioprinted Artificial Trachea with Epithelial Cells and Chondrogenic-Differentiated Bone Marrow-Derived Mesenchymal Stem Cells

Authors
 Sang-Woo Bae  ;  Kang-Woog Lee  ;  Jae-Hyun Park  ;  JunHee Lee  ;  Cho-Rok Jung  ;  JunJie Yu  ;  Hwi-Yool Kim  ;  Dae-Hyun Kim 
Citation
 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol.19(6) : E1624, 2018 
Journal Title
 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 
Issue Date
2018
MeSH
Animals ; Bioprinting*/methods ; Cartilage/metabolism ; Cell Differentiation* ; Chondrogenesis* ; Epithelial Cells/metabolism* ; Glycosaminoglycans/metabolism ; Male ; Mesenchymal Stem Cells/cytology* ; Mesenchymal Stem Cells/metabolism* ; Printing, Three-Dimensional* ; Rabbits ; Tissue Engineering ; Trachea/metabolism*
Keywords
artificial trachea ; bone marrow-derived mesenchymal stem cell ; chondrogenic differentiation ; three-dimensional bioprinting ; tissue engineering
Abstract
Tracheal resection has limited applicability. Although various tracheal replacement strategies were performed using artificial prosthesis, synthetic stents and tissue transplantation, the best method in tracheal reconstruction remains to be identified. Recent advances in tissue engineering enabled 3D bioprinting using various biocompatible materials including living cells, thereby making the product clinically applicable. Moreover, clinical interest in mesenchymal stem cell has dramatically increased. Here, rabbit bone marrow-derived mesenchymal stem cells (bMSC) and rabbit respiratory epithelial cells were cultured. The chondrogenic differentiation level of bMSC cultured in regular media (MSC) and that in chondrogenic media (d-MSC) were compared. Dual cell-containing artificial trachea were manufactured using a 3D bioprinting method with epithelial cells and undifferentiated bMSC (MSC group, n = 6) or with epithelial cells and chondrogenic-differentiated bMSC (d-MSC group, n = 6). d-MSC showed a relatively higher level of glycosaminoglycan (GAG) accumulation and chondrogenic marker gene expression than MSC in vitro. Neo-epithelialization and neo-vascularization were observed in all groups in vivo but neo-cartilage formation was only noted in d-MSC. The epithelial cells in the 3D bioprinted artificial trachea were effective in respiratory epithelium regeneration. Chondrogenic-differentiated bMSC had more neo-cartilage formation potential in a short period. Nevertheless, the cartilage formation was observed only in a localized area.
Files in This Item:
T201805987.pdf Download
DOI
10.3390/ijms19061624
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dae-Hyun(김대현)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/169802
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse