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3D Bioprinted Artificial Trachea with Epithelial Cells and Chondrogenic-Differentiated Bone Marrow-Derived Mesenchymal Stem Cells

DC FieldValueLanguage
dc.contributor.author김대현-
dc.date.accessioned2019-07-11T02:59:01Z-
dc.date.available2019-07-11T02:59:01Z-
dc.date.issued2018-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/169802-
dc.description.abstractTracheal resection has limited applicability. Although various tracheal replacement strategies were performed using artificial prosthesis, synthetic stents and tissue transplantation, the best method in tracheal reconstruction remains to be identified. Recent advances in tissue engineering enabled 3D bioprinting using various biocompatible materials including living cells, thereby making the product clinically applicable. Moreover, clinical interest in mesenchymal stem cell has dramatically increased. Here, rabbit bone marrow-derived mesenchymal stem cells (bMSC) and rabbit respiratory epithelial cells were cultured. The chondrogenic differentiation level of bMSC cultured in regular media (MSC) and that in chondrogenic media (d-MSC) were compared. Dual cell-containing artificial trachea were manufactured using a 3D bioprinting method with epithelial cells and undifferentiated bMSC (MSC group, n = 6) or with epithelial cells and chondrogenic-differentiated bMSC (d-MSC group, n = 6). d-MSC showed a relatively higher level of glycosaminoglycan (GAG) accumulation and chondrogenic marker gene expression than MSC in vitro. Neo-epithelialization and neo-vascularization were observed in all groups in vivo but neo-cartilage formation was only noted in d-MSC. The epithelial cells in the 3D bioprinted artificial trachea were effective in respiratory epithelium regeneration. Chondrogenic-differentiated bMSC had more neo-cartilage formation potential in a short period. Nevertheless, the cartilage formation was observed only in a localized area.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherMDPI-
dc.relation.isPartOfINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBioprinting*/methods-
dc.subject.MESHCartilage/metabolism-
dc.subject.MESHCell Differentiation*-
dc.subject.MESHChondrogenesis*-
dc.subject.MESHEpithelial Cells/metabolism*-
dc.subject.MESHGlycosaminoglycans/metabolism-
dc.subject.MESHMale-
dc.subject.MESHMesenchymal Stem Cells/cytology*-
dc.subject.MESHMesenchymal Stem Cells/metabolism*-
dc.subject.MESHPrinting, Three-Dimensional*-
dc.subject.MESHRabbits-
dc.subject.MESHTissue Engineering-
dc.subject.MESHTrachea/metabolism*-
dc.title3D Bioprinted Artificial Trachea with Epithelial Cells and Chondrogenic-Differentiated Bone Marrow-Derived Mesenchymal Stem Cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Medical Engineering (의학공학교실)-
dc.contributor.googleauthorSang-Woo Bae-
dc.contributor.googleauthorKang-Woog Lee-
dc.contributor.googleauthorJae-Hyun Park-
dc.contributor.googleauthorJunHee Lee-
dc.contributor.googleauthorCho-Rok Jung-
dc.contributor.googleauthorJunJie Yu-
dc.contributor.googleauthorHwi-Yool Kim-
dc.contributor.googleauthorDae-Hyun Kim-
dc.identifier.doi10.3390/ijms19061624-
dc.contributor.localIdA04717-
dc.relation.journalcodeJ01133-
dc.identifier.eissn1422-0067-
dc.identifier.pmid29857483-
dc.subject.keywordartificial trachea-
dc.subject.keywordbone marrow-derived mesenchymal stem cell-
dc.subject.keywordchondrogenic differentiation-
dc.subject.keywordthree-dimensional bioprinting-
dc.subject.keywordtissue engineering-
dc.contributor.alternativeNameKim, Dae-Hyun-
dc.contributor.affiliatedAuthor김대현-
dc.citation.volume19-
dc.citation.number6-
dc.citation.startPageE1624-
dc.identifier.bibliographicCitationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol.19(6) : E1624, 2018-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers

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