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Developing palatal bone using human mesenchymal stem cell and stem cells from exfoliated deciduous teeth cell sheets

Authors
 Jong‐Min Lee  ;  Hyun‐Yi Kim  ;  Jin‐Sung Park  ;  Dong‐Joon Lee  ;  Sushan Zhang  ;  David William Green  ;  Teruo Okano  ;  Jeong‐Ho Hong  ;  Han‐Sung Jung 
Citation
 JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, Vol.13(2) : 319-327, 2019 
Journal Title
 JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE 
ISSN
 1932-6254 
Issue Date
2019
Keywords
SHED ; cell sheet ; cleft palate ; hMSC ; palatal bone ; regenerative medicine
Abstract
Cleft palate is one of the most common craniofacial defects in newborn babies. The characteristics of this genetic disease produce soft and hard tissue defects on the lip and maxilla, which cause not only aesthetic but also functional problems with speech, eating, and breathing. Bone grafts using autologous cancellous bone have been a standard treatment to repair the hard tissue defect in cleft palates. However, such grafts do not fully integrate into host bone and undergo resorption. To overcome engraftment problems, it is common to engineer new tissues with a combination of multipotent cells and biomaterial frameworks. Here, we manufactured cell sheets for bone repair of cleft palates derived from two osteogenic cell sources, human mesenchymal stem cells (hMSCs) and stem cells from human exfoliated deciduous teeth (SHEDs). Cell sheets made from hMSCs and SHEDs gave rise to in vitro calcification, which indicated the osteogenic potential of these cells. The cell sheets of hMSCs and SHEDs expressed the bone-specific osteogenic markers, osterix, osteocalcin, and osteopontin, following insertion into ex vivo-cultured embryonic palatal shelves and in ovo culture. In conclusion, we showed that osteogenic stem cell sheets have mineralization potential and might represent a new alternative to autologous bone transplantation in the reconstruction of cleft palates.
Full Text
https://onlinelibrary.wiley.com/doi/full/10.1002/term.2811
DOI
10.1002/term.2811
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
David, William Green(데이비드 그린)
Lee, Dong Joon(이동준) ORCID logo https://orcid.org/0000-0001-6532-9729
Lee, Jong Min(이종민) ORCID logo https://orcid.org/0000-0002-9466-7644
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/167463
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