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Effective Differentiation of Induced Pluripotent Stem Cells Into Dental Cells

Authors
 Eun‐Jung Kim  ;  Kyung‐Sik Yoon  ;  Makiko Arakaki  ;  Keishi Otsu  ;  Satoshi Fukumoto  ;  Hidemitsu Harada  ;  David William Green  ;  Jong‐Min Lee  ;  Han‐Sung Jung 
Citation
 DEVELOPMENTAL DYNAMICS, Vol.248(1) : 129-139, 2019 
Journal Title
 DEVELOPMENTAL DYNAMICS 
ISSN
 1058-8388 
Issue Date
2019
Keywords
biotooth ; dental epithelial cells ; dental mesenchymal cells ; iPSCs ; recombinations ; transwell
Abstract
BACKGROUND: A biotooth is defined as a complete living tooth, made in laboratory cultures from a spontaneous interplay between epithelial and mesenchymal cell-based frontal systems. A good solution to these problems is to use induced pluripotent stem cells (iPSCs). However, no one has yet formulated culture conditions that effectively differentiate iPSCs into dental epithelial and dental mesenchymal cells phenotypes analogous to those present in tooth development. RESULTS: Here, we tried to induce differentiation methods for dental epithelial cells (DEC) and dental mesenchymal cells from iPSCs. For the DEC differentiation, the conditional media of SF2 DEC was adjusted to embryoid body. Moreover, we now report on a new cultivation protocol, supported by transwell membrane cell culture that make it possible to differentiate iPSCs into dental epithelial and mesenchymal cells with abilities to initiate the first stages in de novo tooth formation. CONCLUSIONS: Implementation of technical modifications to the protocol that maximize the number and rate of iPSC differentiation, into mesenchymal and epithelial cell layers, will be the next step toward growing an anatomically accurate biomimetic tooth organ.
Full Text
https://onlinelibrary.wiley.com/doi/full/10.1002/dvdy.24663
DOI
10.1002/dvdy.24663
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eun-Jung(김은정) ORCID logo https://orcid.org/0000-0002-9515-7590
David, William Green(데이비드 그린)
Lee, Jong Min(이종민) ORCID logo https://orcid.org/0000-0002-9466-7644
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/167321
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