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Lipidomic differentiation of Graves' ophthalmopathy in plasma and urine from Graves' disease patients.

 Seul Kee Byeon  ;  Se Hee Park  ;  Jong Cheol Lee  ;  Sena Hwang  ;  Cheol Ryong Ku  ;  Dong Yeob Shin  ;  Jin Sook Yoon  ;  Eun Jig Lee  ;  Myeong Hee Moon 
 Analytical and Bioanalytical Chemistry, Vol.410(27) : 7121-7133, 2018 
Journal Title
 Analytical and Bioanalytical Chemistry 
Issue Date
Chromatography, High Pressure Liquid/methods ; Female ; Graves Ophthalmopathy/blood* ; Graves Ophthalmopathy/diagnosis ; Graves Ophthalmopathy/metabolism ; Graves Ophthalmopathy/urine* ; Humans ; Lipid Metabolism ; Lipids/blood* ; Lipids/urine* ; Male ; Metabolomics/methods ; Spectrometry, Mass, Electrospray Ionization/methods ; Tandem Mass Spectrometry/methods
Graves’ disease ; Lipidomic analysis ; Ophthalmopathy ; Plasma ; Urine ; nUPLC-ESI-MS/MS
Approximately 50% of patients with Graves' disease (GD) develop retracted eyelids with bulging eyes, known as Graves' ophthalmopathy (GO). However, no simple diagnostic blood marker for distinguishing GO from GD has been developed yet. The objective of this study was to conduct comprehensive profiling of lipids using plasma and urine samples from patients with GD and GO undergoing antithyroid therapy using nanoflow ultrahigh performance liquid chromatography electrospray ionization tandem mass spectrometry. Plasma (n = 86) and urine (n = 75) samples were collected from 23 patients with GD without GO, 31 patients with GO, and 32 healthy controls. Among 389 plasma and 273 urinary lipids that were structurally identified, 281 plasma and 191 urinary lipids were quantified in selected reaction monitoring mode. High-abundance lipids were significantly altered, indicating that the development of GD is evidently related to altered lipid metabolism in both plasma and urine. Several urinary lysophosphatidylcholine species were found to be increased (3- to 10-fold) in both GD and GO. While the overall lipid profiles between GD and GO were similar, significant changes (area under receiver operating curve > 0.8) in GO vs. GD were observed in a few lipid profiles: 58:7-TG and (16:1,18:0)-DG from plasma, 16:1-PC and 50:1-TG from urine, and d18:1-S1P from both plasma and urine samples. An altered metabolism of lipids associated with the additional development of ophthalmopathy was confirmed with the discovery of several candidate markers. These can be suggested as candidate markers for differentiating the state of GO and GD patients based on plasma or urinary lipidomic analysis. Graphical abstract.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
구철룡(Ku, Cheol Ryong) ORCID logo https://orcid.org/0000-0001-8693-9630
신동엽(Shin, Dong Yeob) ORCID logo https://orcid.org/0000-0003-1048-7978
윤진숙(Yoon, Jin Sook) ORCID logo https://orcid.org/0000-0002-8751-9467
이은직(Lee, Eun Jig) ORCID logo https://orcid.org/0000-0002-9876-8370
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