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Effect of Polydeoxyribonucleotide on Angiogenesis and Wound Healing in an In Vitro Model of Osteoarthritis.

Authors
 Ahreum Baek  ;  Yoon Kim  ;  Jin Woo Lee  ;  Sang Chul Lee  ;  Sung-Rae Cho 
Citation
 Cell Transplantation, Vol.27(11) : 1623-1633, 2018 
Journal Title
 Cell Transplantation 
ISSN
 0963-6897 
Issue Date
2018
Keywords
anabolism ; angiogenesis ; catabolism ; osteoarthritis ; polydeoxyribonucleotide ; wound healing
Abstract
Osteoarthritis (OA) is degenerative disease, leading to pain and functional disability. It is reported that polydeoxyribonucleotide (PDRN) is a suitable therapy for OA. However, the therapeutic mechanisms of PDRN in OA are not fully understood. To investigate the effect of PDRN in an in vitro model of OA, interleukin (IL)-1β or phosphate-buffered saline (PBS) was used to treat a human chondrocytic cell line in hypoxic conditions for 24 h (IL-1β group or control group). PDRN was then used to treat IL-1β group cells for 24 h (PDRN group). By Label-Based Human Antibody Array 1000, angiopoietin-2 (ANG-2), platelet-derived growth factor (PDGF), angiostatin, and endostatin, which were related to angiogenesis, were chosen for further validation studies. Quantitative real-time reverse transcription polymerase chain reaction and western blot analysis validated that the levels of PDGF and ANG-2, which were related to pro-angiogenesis, were significantly increased in the PDRN group compared with those in the control group or the IL-1β group. However, the levels of endostatin and angiostatin, which were related in anti-angiogenesis, were significantly decreased in the PDRN group compared with those in the control group or the IL-1β group. In the same manner, vascular endothelial growth factor, which was a mediator of angiogenesis, was significantly increased in the PDRN group compared with those in the control group or the IL-1β group. Furthermore, wound closure was significantly increased in the PDRN group compared with the control group or the IL-1β group by in vitro scratch assay. Moreover, PDRN decreased expression of metalloproteinase 13, as a catabolic factor for OA, but increased expression of aggrecan, which was an anabolic factor for OA. These data suggest that PDRN may promote angiogenesis and wound healing via down-regulation of catabolism and up-regulation of anabolism in an in vitro model of OA.
Full Text
https://journals.sagepub.com/doi/full/10.1177/0963689718804130
DOI
10.1177/0963689718804130
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Rehabilitation Medicine (재활의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
이상철(Lee, Sang Chul) ORCID logo https://orcid.org/0000-0002-6241-7392
이진우(Lee, Jin Woo) ORCID logo https://orcid.org/0000-0002-0293-9017
조성래(Cho, Sung-Rae) ORCID logo https://orcid.org/0000-0003-1429-2684
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/165373
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