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Enhancing proliferation and optimizing the culture condition for human bone marrow stromal cells using hypoxia and fibroblast growth factor-2.

Authors
 Jung-Seok Lee  ;  Seul Ki Kim  ;  Byung-Joo Jung  ;  Seong-Bok Choi  ;  Eun-Young Choi  ;  Chang-Sung Kim 
Citation
 STEM CELL RESEARCH, Vol.28 : 87-95, 2018 
Journal Title
 STEM CELL RESEARCH 
ISSN
 1873-5061 
Issue Date
2018
Keywords
Bone marrow stromal cells ; Fibroblast growth factor ; Hypoxia ; Microenvironment interactions ; Tissue regeneration
Abstract
This study aimed to determine the cellular characteristics and behaviors of human bone marrow stromal cells (hBMSCs) expanded in media in a hypoxic or normoxic condition and with or without fibroblast growth factor-2 (FGF-2) treatment. hBMSCs isolated from the vertebral body and expanded in these four groups were evaluated for cellular proliferation/migration, colony-forming units, cell-surface characterization, in vitro differentiation, in vivo transplantation, and gene expression. Culturing hBMSCs using a particular environmental factor (hypoxia) and with the addition of FGF-2 increased the cellular proliferation rate while enhancing the regenerative potential, modulated the multipotency-related processes (enhanced chondrogenesis-related processes/osteogenesis, but reduced adipogenesis), and increased cellular migration and collagen formation. The gene expression levels in the experimental samples showed activation of the hypoxia-inducible factor-1 pathway and glycolysis in the hypoxic condition, with this not being affected by the addition of FGF-2. The concurrent application of hypoxia and FGF-2 could provide a favorable condition for culturing hBMSCs to be used in clinical applications associated with bone tissue engineering, due to the enhancement of cellular proliferation and regenerative potential.
Files in This Item:
T201803686.pdf Download
DOI
10.1016/j.scr.2018.01.010
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Sung(김창성) ORCID logo https://orcid.org/0000-0003-3902-1071
Lee, Jung Seok(이중석) ORCID logo https://orcid.org/0000-0003-1276-5978
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/165246
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