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Enhancing proliferation and optimizing the culture condition for human bone marrow stromal cells using hypoxia and fibroblast growth factor-2.

DC Field Value Language
dc.contributor.author김창성-
dc.contributor.author이중석-
dc.date.accessioned2018-11-16T16:42:32Z-
dc.date.available2018-11-16T16:42:32Z-
dc.date.issued2018-
dc.identifier.issn1873-5061-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/165246-
dc.description.abstractThis study aimed to determine the cellular characteristics and behaviors of human bone marrow stromal cells (hBMSCs) expanded in media in a hypoxic or normoxic condition and with or without fibroblast growth factor-2 (FGF-2) treatment. hBMSCs isolated from the vertebral body and expanded in these four groups were evaluated for cellular proliferation/migration, colony-forming units, cell-surface characterization, in vitro differentiation, in vivo transplantation, and gene expression. Culturing hBMSCs using a particular environmental factor (hypoxia) and with the addition of FGF-2 increased the cellular proliferation rate while enhancing the regenerative potential, modulated the multipotency-related processes (enhanced chondrogenesis-related processes/osteogenesis, but reduced adipogenesis), and increased cellular migration and collagen formation. The gene expression levels in the experimental samples showed activation of the hypoxia-inducible factor-1 pathway and glycolysis in the hypoxic condition, with this not being affected by the addition of FGF-2. The concurrent application of hypoxia and FGF-2 could provide a favorable condition for culturing hBMSCs to be used in clinical applications associated with bone tissue engineering, due to the enhancement of cellular proliferation and regenerative potential.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.languageEnglish-
dc.publisherElsevier-
dc.relation.isPartOfSTEM CELL RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleEnhancing proliferation and optimizing the culture condition for human bone marrow stromal cells using hypoxia and fibroblast growth factor-2.-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry (치과대학)-
dc.contributor.departmentDept. of Periodontics (치주과학교실)-
dc.contributor.googleauthorJung-Seok Lee-
dc.contributor.googleauthorSeul Ki Kim-
dc.contributor.googleauthorByung-Joo Jung-
dc.contributor.googleauthorSeong-Bok Choi-
dc.contributor.googleauthorEun-Young Choi-
dc.contributor.googleauthorChang-Sung Kim-
dc.identifier.doi10.1016/j.scr.2018.01.010-
dc.contributor.localIdA01041-
dc.contributor.localIdA03185-
dc.relation.journalcodeJ02680-
dc.identifier.eissn1876-7753-
dc.identifier.pmid29448134-
dc.subject.keywordBone marrow stromal cells-
dc.subject.keywordFibroblast growth factor-
dc.subject.keywordHypoxia-
dc.subject.keywordMicroenvironment interactions-
dc.subject.keywordTissue regeneration-
dc.contributor.alternativeNameKim, Chang Sung-
dc.contributor.alternativeNameLee, Jung Seok-
dc.contributor.affiliatedAuthor김창성-
dc.contributor.affiliatedAuthor이중석-
dc.citation.volume28-
dc.citation.startPage87-
dc.citation.endPage95-
dc.identifier.bibliographicCitationSTEM CELL RESEARCH, Vol.28 : 87-95, 2018-
dc.identifier.rimsid58661-
dc.type.rimsART-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers

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