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Time-Lapse Live-Cell Imaging Reveals Dual Function of Oseg4, Drosophila WDR35, in Ciliary Protein Trafficking

Authors
 Nayoung Lee  ;  Jina Park  ;  Yong Chul Bae  ;  Jung Ho Lee  ;  Chul Hoon Kim  ;  Seok Jun Moon 
Citation
 MOLECULES AND CELLS, Vol.41(7) : 676-683, 2018 
Journal Title
 MOLECULES AND CELLS 
ISSN
 1016-8478 
Issue Date
2018
Keywords
Drosophila ; Oseg4 ; intraflagellar transport ; live imaging ; primary cilia
Abstract
Cilia are highly specialized antennae-like organelles that extend from the cell surface and act as cell signaling hubs. Intraflagellar transport (IFT) is a specialized form of intracellular protein trafficking that is required for the assembly and maintenance of cilia. Because cilia are so important, mutations in several IFT components lead to human disease. Thus, clarifying the molecular functions of the IFT proteins is a high priority in cilia biology. Live imaging in various species and cellular preparations has proven to be an important technique in both the discovery of IFT and the mechanisms by which it functions. Live imaging of Drosophila cilia, however, has not yet been reported. Here, we have visualized the movement of IFT in Drosophila cilia using time-lapse live imaging for the first time. We found that NOMPB-GFP (IFT88) moves according to distinct parameters depending on the ciliary segment. NOMPB-GFP moves at a similar speed in proximal and distal cilia toward the tip (~0.45 μm/s). As it returns to the ciliary base, however, NOMPB-GFP moves at ~0.12 μm/s in distal cilia, accelerating to ~0.70 μm/s in proximal cilia. Furthermore, while live imaging NOMPB-GFP, we observed one of the IFT proteins required for retrograde movement, Oseg4 (WDR35), is also required for anterograde movement in distal cilia. We anticipate our time-lapse live imaging analysis technique in Drosophila cilia will be a good starting point for a more sophisticated analysis of IFT and its molecular mechanisms.
Files in This Item:
T201802615.pdf Download
DOI
10.14348/molcells.2018.0179
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chul Hoon(김철훈) ORCID logo https://orcid.org/0000-0002-7360-429X
Moon, Seok Jun(문석준) ORCID logo https://orcid.org/0000-0001-7282-2888
Lee, Jeong Ho(이정호)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/163242
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