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Role of microRNA-146a in regulation of fibrosis in orbital fibroblasts from patients with Graves' orbitopathy

Authors
 Sun Young Jang  ;  Seong Jun Park  ;  Min Kyung Chae  ;  Joon H Lee  ;  Eun Jig Lee  ;  Jin Sook Yoon 
Citation
 British Journal of Ophthalmology, Vol.102(3) : 407-414, 2018 
Journal Title
 British Journal of Ophthalmology 
ISSN
 0007-1161 
Issue Date
2018
Keywords
experimental laboratory
Abstract
AIM: To examine the role of microRNA-146a (miR-146a) in the regulation of fibrosis in an in vitro model of Graves' orbitopathy (GO). METHODS: Orbital fat/connective tissues were harvested from patients with GO and non-GO for primary orbital fibroblast cultures. The effects of transforming growth factor-beta (TGF-beta), a potent cytokine that promotes fibrosis, on miR-146a expression were analysed in GO and non-GO orbital fibroblasts using quantitative real-time PCR. The effects of overexpressed miR-146a on TGF-beta-induced fibrotic markers were examined in GO orbital fibroblasts by western blot analysis. Expression ofSma and Mad related family (Smad) 4/tumour necrosis factor receptor-associated factor 6 (TRAF6) after transfection of miR-146a mimics or inhibitors were examined. RESULTS: TGF-beta induced an increase in miR-146a expression in orbital fibroblasts from patients with GO in a time-dependent and concentration-dependent manner. miR-146a mimics further decreased the production of TGF-beta-induced fibronectin, collagen Ialpha and alpha-smooth muscle actin protein. The Smad4 and TRAF6 protein levels were significantly decreased by miR-146a mimics, compared with control mimics, and significantly increased on inhibition of miR-146a production compared with a control. CONCLUSIONS: miR-146a plays a role as a negative regulator in the production of TGF-beta-induced fibrotic markers. Thus, miR-146a may be involved in the regulation of fibrosis in orbital fibroblasts from patients with GO.
Full Text
https://bjo.bmj.com/content/102/3/407
DOI
10.1136/bjophthalmol-2017-310723
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실)
Yonsei Authors
윤진숙(Yoon, Jin Sook) ORCID logo https://orcid.org/0000-0002-8751-9467
이은직(Lee, Eun Jig) ORCID logo https://orcid.org/0000-0002-9876-8370
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/162664
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