0 96

Cited 1 times in

Role of microRNA-146a in regulation of fibrosis in orbital fibroblasts from patients with Graves' orbitopathy

DC FieldValueLanguage
dc.contributor.author윤진숙-
dc.contributor.author이은직-
dc.date.accessioned2018-08-28T17:29:01Z-
dc.date.available2018-08-28T17:29:01Z-
dc.date.issued2018-
dc.identifier.issn0007-1161-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/162664-
dc.description.abstractAIM: To examine the role of microRNA-146a (miR-146a) in the regulation of fibrosis in an in vitro model of Graves' orbitopathy (GO). METHODS: Orbital fat/connective tissues were harvested from patients with GO and non-GO for primary orbital fibroblast cultures. The effects of transforming growth factor-beta (TGF-beta), a potent cytokine that promotes fibrosis, on miR-146a expression were analysed in GO and non-GO orbital fibroblasts using quantitative real-time PCR. The effects of overexpressed miR-146a on TGF-beta-induced fibrotic markers were examined in GO orbital fibroblasts by western blot analysis. Expression ofSma and Mad related family (Smad) 4/tumour necrosis factor receptor-associated factor 6 (TRAF6) after transfection of miR-146a mimics or inhibitors were examined. RESULTS: TGF-beta induced an increase in miR-146a expression in orbital fibroblasts from patients with GO in a time-dependent and concentration-dependent manner. miR-146a mimics further decreased the production of TGF-beta-induced fibronectin, collagen Ialpha and alpha-smooth muscle actin protein. The Smad4 and TRAF6 protein levels were significantly decreased by miR-146a mimics, compared with control mimics, and significantly increased on inhibition of miR-146a production compared with a control. CONCLUSIONS: miR-146a plays a role as a negative regulator in the production of TGF-beta-induced fibrotic markers. Thus, miR-146a may be involved in the regulation of fibrosis in orbital fibroblasts from patients with GO.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherBMJ Pub. Group-
dc.relation.isPartOfBritish Journal of Ophthalmology-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleRole of microRNA-146a in regulation of fibrosis in orbital fibroblasts from patients with Graves' orbitopathy-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Ophthalmology-
dc.contributor.googleauthorSun Young Jang-
dc.contributor.googleauthorSeong Jun Park-
dc.contributor.googleauthorMin Kyung Chae-
dc.contributor.googleauthorJoon H Lee-
dc.contributor.googleauthorEun Jig Lee-
dc.contributor.googleauthorJin Sook Yoon-
dc.identifier.doi10.1136/bjophthalmol-2017-310723-
dc.contributor.localIdA02611-
dc.contributor.localIdA03050-
dc.relation.journalcodeJ00412-
dc.identifier.eissn1468-2079-
dc.identifier.pmid29101123-
dc.identifier.urlhttps://bjo.bmj.com/content/102/3/407-
dc.subject.keywordexperimental laboratory-
dc.contributor.alternativeNameYoon, Jin Sook-
dc.contributor.alternativeNameLee, Eun Jig-
dc.contributor.affiliatedAuthorYoon, Jin Sook-
dc.contributor.affiliatedAuthorLee, Eun Jig-
dc.citation.volume102-
dc.citation.number3-
dc.citation.startPage407-
dc.citation.endPage414-
dc.identifier.bibliographicCitationBritish Journal of Ophthalmology, Vol.102(3) : 407-414, 2018-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.