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Cell cycle of the enamel knot during tooth morphogenesis

Authors
 Seo-Yoon Jung  ;  David William Green  ;  Han-Sung Jung  ;  Eun-Jung Kim 
Citation
 Histochemistry and Cell Biology, Vol.149(6) : 655-659, 2018 
Journal Title
 Histochemistry and Cell Biology 
ISSN
 0948-6143 
Issue Date
2018
MeSH
Animals ; *Cell Cycle ; Dental Enamel/*cytology/embryology/*metabolism ; Epithelium/metabolism ; Gerbillinae ; Mice ; Inbred ICR Mice ; *Morphogenesis ; Tooth/*cytology/embryology/*metabolism
Keywords
Cell cycle ; Cusp ; Enamel knot ; Gerbil ; Mouse ; Tooth morphogenesis
Abstract
Enamel knot (EK) is known to be a central organ in tooth development, especially for cusp patterning. To trace the exact position and movement among the inner dental epithelium (IDE) and EK cells, and to monitor the relationship between the EK and cusp patterning, it is essential that we understand the cell cycle status of the EK in early stages of tooth development. In this study, thymidine analogous (IdU, BrdU) staining was used to evaluate the cell cycle phase of the primary EK at the early casp stage (E13.0) and the gerbil embryo (E19) in a developing mouse embryo. The centerpiece of this study was to describe the cell cycle phasing and sequencing during proliferation in the IDE according to the expression of IdU and BrdU following their injection at calculated time points. The interval time between IdU injection and BrdU injection was set at 4 h. As a result, the cell cycle in the IDE of the mouse and gerbil was found to be synchronous. Conversely, the cell cycle in primary EKs of mice was much longer than that of the IDE. Therefore, the difference of cell cycle of the IDE and the EK is related to the diversity of cusp patterning and would provide a new insight into tooth morphogenesis.
Full Text
https://link.springer.com/article/10.1007%2Fs00418-018-1666-9
DOI
10.1007/s00418-018-1666-9
Appears in Collections:
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실)
Yonsei Authors
정한성(Jung, Han Sung)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/162506
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