28 32

The Effect of Environmental Enrichment on Glutathione-Mediated Xenobiotic Metabolism and Antioxidation in Normal Adult Mice

Authors
 Jung Hwa Seo  ;  Soonil Pyo  ;  Yoon-Kyum Shin  ;  Bae-Geun Nam  ;  Jeong Won Kang  ;  Kwang Pyo Kim  ;  Hoo Young Lee  ;  Sung-Rae Cho 
Citation
 Frontiers in Neurology, Vol.9 : 425, 2018 
Journal Title
 Frontiers in Neurology 
Issue Date
2018
Keywords
antioxidant ; detoxification ; environmental enrichment ; glutathione ; metabolizing enzymes ; olfactory bulb
Abstract
Olfactory bulb (OB) plays an important role in protecting against harmful substances via the secretion of antioxidant and detoxifying enzymes. Environmental enrichment (EE) is a common rehabilitation method and known to have beneficial effects in the central nervous system. However, the effects of EE in the OB still remain unclear. At 6 weeks of age, CD-1(R) (ICR) mice were assigned to standard cages or EE cages. After 2 months, we performed proteomic analysis. Forty-four up-regulated proteins were identified in EE mice compared to the control mice. Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes Pathway demonstrated that the upregulated proteins were mainly involved in metabolic pathways against xenobiotics. Among those upregulated proteins, 9 proteins, which participate in phase I or II of the xenobiotic metabolizing process and are known to be responsible for ROS detoxification, were validated by qRT-PCR. To explore the effect of ROS detoxification mediated by EE, glutathione activity was measured by an ELISA assay. The ratio of reduced glutathione to oxidized glutathione was significantly increased in EE mice. Based on a linear regression analysis, GSTM2 and UGT2A1 were found to be the most influential genes in ROS detoxification. For further analysis of neuroprotection, the level of iNOS and the ratio of Bax to Bcl-2 were significantly decreased in EE mice. While TUNEL(+) cells were significantly decreased, Ki67(+) cells were significantly increased in EE mice, implicating that EE creates an optimal state for xenobiotic metabolism and antioxidant activity. Taken together, our results suggested that EE protects olfactory layers via the upregulation of glutathione-related antioxidant and xenobiotic metabolizing enzymes, eventually lowering ROS-mediated inflammation and apoptosis and increasing neurogenesis. This study may provide an opportunity for a better understanding of the beneficial effects of EE in the OB.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/162496
Files in This Item:
T201801770.pdf Download
DOI
10.3389/fneur.2018.00425
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Rehabilitation Medicine (재활의학교실)
Yonsei Authors
조성래(Cho, Sung-Rae) ORCID logo https://orcid.org/0000-0003-1429-2684
Export
RIS (EndNote)
XLS (Excel)
XML
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse