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The Differential Effects of Repetitive Magnetic Stimulation in an In Vitro Neuronal Model of Ischemia/Reperfusion Injury

Authors
 Ahreum Baek  ;  Ji Hyun Kim  ;  Soonil Pyo  ;  Joon-Ho Jung  ;  Eun Jee Park  ;  Sung Hoon Kim  ;  Sung-Rae Cho 
Citation
 Frontiers in Neurology, Vol.9 : 50, 2018 
Journal Title
 Frontiers in Neurology 
Issue Date
2018
Keywords
Ca2+-calmodulin-dependent protein kinase II-cAMP-response element binding protein signaling pathway ; apoptosis ; brain-derived neurotrophic factor ; extracellular signal-regulated kinases and AKT signaling pathway ; high frequency ; in vitro neuronal model of ischemia/reperfusion injury ; repetitive magnetic stimulation ; synaptic plasticity
Abstract
Repetitive transcranial magnetic stimulation (rTMS) is a non-invasive therapy that has been implicated in treatment of serious neurological disorders. However, the neurobiological mechanisms underlying the effects of rTMS remain unclear. Therefore, this study examined the differential effects of repetitive magnetic stimulation (rMS) in an in vitro neuronal model of ischemia/reperfusion (I/R) injury, depending on low and high frequency. Neuro-2a cells were differentiated with retinoic acid and established for in vitro neuronal model of I/R injury under a subsequent 3 h of oxygen and glucose deprivation/reoxygenation (OGD/R) condition. After the I/R injury, the differentiated neuronal cells were stimulated with rMS on day 1 and randomly divided into three groups: OGD/R+sham, OGD/R+low-frequency, and OGD/R+high-frequency groups. High-frequency rMS increases cell proliferation through activation of extracellular signal-regulated kinases and AKT-signaling pathway and inhibits apoptosis in OGD/R-injured cells. Furthermore, high-frequency rMS increases Ca(2+)-calmodulin-dependent protein kinase II (CaMKII)-cAMP-response element binding protein (CREB) signaling pathway, further leading to alternation of brain-derived neurotrophic factor expression and synaptic plasticity in OGD/R injured cells. These results verified the neurobiological mechanisms of frequency-dependent rMS in I/R injury-treated neuronal cells. These mechanisms will help develop more powerful and credible rTMS stimulation treatment protocols.
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DOI
10.3389/fneur.2018.00050
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Rehabilitation Medicine (재활의학교실) > 1. Journal Papers
Yonsei Authors
정준호(Chung, Joon Ho)
조성래(Cho, Sung-Rae) ORCID logo https://orcid.org/0000-0003-1429-2684
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/161946
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