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Aptamer-functionalized capacitance sensors for real-time monitoring of bacterial growth and antibiotic susceptibility

Authors
 Namgyeong Jo  ;  Bongjun Kim  ;  Sun-Mi Lee  ;  Jeseung Oh  ;  In Ho Park  ;  Kook Jin Lim  ;  Jeon-Soo Shin  ;  Kyung-Hwa Yoo 
Citation
 Biosensors & Bioelectronics, Vol.102 : 164-170, 2018 
Journal Title
 Biosensors & Bioelectronics 
ISSN
 0956-5663 
Issue Date
2018
Keywords
Antibiotic susceptibility testing ; Aptamer ; Bacterial growth ; Capacitance sensor ; Identification
Abstract
To prevent spread of infection and antibiotic resistance, fast and accurate diagnosis of bacterial infection and subsequent administration of antimicrobial agents are important. However, conventional methods for bacterial detection and antibiotic susceptibility testing (AST) require more than two days, leading to delays that have contributed to an increase in antibiotic-resistant bacteria. Here, we report an aptamer-functionalized capacitance sensor array that can monitor bacterial growth and antibiotic susceptibility in real-time. While E. coli and S. aureus were cultured, the capacitance increased over time, and apparent bacterial growth curves were observed even when 10 CFU/mL bacteria was inoculated. Furthermore, because of the selectivity of aptamers, bacteria could be identified within 1h using the capacitance sensor array functionalized with aptamers. In addition to bacterial growth, antibiotic susceptibility could be monitored in real-time. When bacteria were treated with antibiotics above the minimum inhibitory concentration (MIC), the capacitance decreased because the bacterial growth was inhibited. These results demonstrate that the aptamer-functionalized capacitance sensor array might be applied for rapid ASTs.
Full Text
https://www.sciencedirect.com/science/article/pii/S0956566317307236
DOI
10.1016/j.bios.2017.11.010
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
박인호(Park, Inho) ORCID logo https://orcid.org/0000-0003-2190-5469
신전수(Shin, Jeon Soo) ORCID logo https://orcid.org/0000-0002-8294-3234
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/161828
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