0 15

Cited 0 times in

Spatiotemporal expression patterns of clusterin in the mouse inner ear

Authors
 Seokwon Lee  ;  Jeong-Oh Shin  ;  Borum Sagong  ;  Un-Kyung Kim  ;  Jinwoong Bok 
Citation
 Cell and Tissue Research, Vol.370(1) : 89-97, 2017 
Journal Title
 Cell and Tissue Research 
ISSN
 0302-766X 
Issue Date
2017
Keywords
Clusterin ; Expression ; Inner ear ; Mouse ; Spatiotemporal
Abstract
Clusterin (CLU) is an extracellular chaperone protein that is implicated in diverse physiological and pathophysiological cellular processes. CLU expression is upregulated in response to cellular stress and under certain conditions, such as neurodegenerative disease and cancer. CLU primarily functions as a chaperone that exerts cytoprotective effects by removing cellular debris and misfolded proteins and also acts as a signaling molecule that regulates pro-survival pathways. Deafness is caused by genetic factors and various extrinsic insults, including ototoxic drugs, exposure to loud sounds and aging. Considering its cytoprotectivity, CLU may also mediate cellular defense mechanisms against hearing loss due to cellular stresses. To understand the function of CLU in the inner ear, we analyze CLU expression patterns in the mouse inner ear during development and in the adult stage. Results of quantitative real-time polymerase chain reaction analysis showed that Clu mRNA levels in the inner ear were increased during embryogenesis and were constantly expressed in the adult. Detailed spatial expression patterns of Clu both in the mRNA and protein levels were analyzed throughout various developmental stages via in situ hybridization and immunofluorescence staining. Clu expression was found in specific domains of developing inner ear starting from the otocyst stage, mainly adjacent to the prosensory domain of the cochlear epithelium. In the mature inner ear, Clu expression was observed in Deiter's cells and pillar cells of the organ of Corti, outer sulcus and in basal cells of the stria vascularis in the cochlea. These specific spatiotemporal expression patterns suggest the possible roles of CLU in inner ear development and in maintaining proper hearing function.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/161322
DOI
10.1007/s00441-017-2650-8
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실)
Yonsei Authors
복진웅(Bok, Jin Woong) ; 신정오(Shin, Jeong Oh)
사서에게 알리기
  feedback
Full Text
https://link.springer.com/article/10.1007%2Fs00441-017-2650-8
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse