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Scar Prevention and Enhanced Wound Healing Induced by Polydeoxyribonucleotide in a Rat Incisional Wound-Healing Model

 Woonhyeok Jeong  ;  Chae Eun Yang  ;  Tai Suk Roh  ;  Jun Hyung Kim  ;  Ju Hee Lee  ;  Won Jai Lee 
 International Journal of Molecular Sciences, Vol.18(8) : E1968, 2017 
Journal Title
 International Journal of Molecular Sciences 
Issue Date
Animals ; Cicatrix/metabolism ; Cicatrix/pathology ; Cicatrix/prevention & control* ; Collagen Type I/biosynthesis ; Collagen Type III/biosynthesis ; Disease Models, Animal ; HMGB1 Protein/metabolism ; Male ; Polydeoxyribonucleotides/pharmacology* ; Rats ; Rats, Sprague-Dawley ; Wound Healing/drug effects* ; Wounds and Injuries/drug therapy* ; Wounds and Injuries/metabolism ; Wounds and Injuries/pathology
cicatrix, inflammation ; polydeoxyribonucleotide ; rats ; wounds and injuries
High-mobility group box protein-1 (HMGB-1) plays a central role in the inflammatory network, and uncontrolled chronic inflammation can lead to excessive scarring. The aim of this study was to evaluate the anti-inflammatory effects of polydeoxyribonucleotide (PDRN) on scar formation. Sprague-Dawley rats (n = 30) underwent dorsal excision of the skin, followed by skin repair. PDRN (8 mg/kg) was administered via intraperitoneal injection for three (PDRN-3 group, n = 8) or seven (PDRN-7 group, n = 8) days, and HMGB-1 was administered via intradermal injection in addition to PDRN treatment for three days (PDRN-3+HMGB-1 group; n = 6). The scar-reducing effects of PDRN were evaluated in the internal scar area and by inflammatory cell counts using histology and immunohistochemistry. Western blot, immunohistochemistry and immunofluorescence assays were performed to observe changes in type I and type III collagen and the expression of HMGB-1 and CD45. Treatment with PDRN significantly reduced the scar area, inflammatory cell infiltration and the number of CD45-positive cells. In addition, the increased expression of HMGB-1 observed in the sham group was significantly reduced after treatment with PDRN. Rats administered HMGB-1 in addition to PDRN exhibited scar areas with inflammatory cell infiltration similar to the sham group, and the collagen synthesis effects of PDRN were reversed. In summary, PDRN exerts anti-inflammatory and collagen synthesis effects via HMGB-1 suppression, preventing scar formation. Thus, we believe that the anti-inflammatory and collagen synthesis effects of PDRN resulted in faster wound healing and decreased scar formation.
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1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jihee(김지희) ORCID logo https://orcid.org/0000-0002-0047-5941
Yang, Chae Eun(양채은) ORCID logo https://orcid.org/0000-0001-8128-791X
Lee, Won Jai(이원재) ORCID logo https://orcid.org/0000-0003-3056-0503
Lee, Ju Hee(이주희) ORCID logo https://orcid.org/0000-0002-1739-5956
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