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Effect of Anti-vascular Endothelial Growth Factor Antibody on the Survival of Cultured Retinal Ganglion Cells

 Ji Min Lee  ;  Hyoung Won Bae  ;  Sang Yeop Lee  ;  Gong Je Seong  ;  Chan Yun Kim 
 Korean Journal of Ophthalmology, Vol.31(4) : 360-365, 2017 
Journal Title
 Korean Journal of Ophthalmology 
Issue Date
Angiogenesis Inhibitors/pharmacology ; Animals ; Antibodies/pharmacology* ; Bevacizumab/pharmacology ; Blotting, Western ; Cell Differentiation ; Cell Survival ; Cells, Cultured ; Gene Expression Regulation/drug effects ; Mice ; Oxidative Stress/genetics* ; RNA/genetics* ; Real-Time Polymerase Chain Reaction ; Retinal Ganglion Cells/cytology* ; Vascular Endothelial Growth Factor A/biosynthesis ; Vascular Endothelial Growth Factor A/genetics ; Vascular Endothelial Growth Factor A/immunology*
Anti-vascular endothelial growth factor ; Bevacizumab ; Oxidative stress ; RGC-5 ; Retinal ganglion cell
PURPOSE: To investigate the effects of anti-vascular endothelial growth factor (VEGF) antibody on the survival of retinal ganglion cell (RGC)-5 cells differentiated with staurosporine under oxidative stress. METHODS: We used real-time polymerase chain reaction and Western blot to confirm the expression of VEGF, VEGF receptor (VEGFR)-1 and VEGFR-2 in RGC-5 cells differentiated with staurosporine for 6 hours. The differentiated RGC-5 cells were treated with 800 μM hydrogen peroxide (H₂O₂) for 24 hours to induce oxidative stress. Then, the survival rate of RGC-5 was confirmed by lactate dehydrogenase assay at each concentration (0, 0.01, 0.1, and 1 mg) using bevacizumab as the anti-VEGF antibody. The expression of VEGF, VEGFR-1, and VEGFR-2 was confirmed using real-time polymerase chain reaction. RESULTS: VEGF, VEGFR-1, and VEGFR-2 were all expressed in differentiated RGC-5 cells. When RGC-5 cells were simultaneously treated with bevacizumab and 800 μM H₂O₂, survival of RGC-5 decreased with bevacizumab concentration. VEGF expression in RGC-5 cells increased with increasing concentration of bevacizumab. Similar patterns were observed for VEGFR-1 and VEGFR-2, but the degree of increase was smaller than that for VEGF. CONCLUSIONS: When bevacizumab was administered to differentiated RGC-5 cells, the cell damage caused by oxidative stress increased. Therefore, given these in vitro study results, caution should be exercised with bevacizumab treatment.
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1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chan Yun(김찬윤) ORCID logo https://orcid.org/0000-0002-8373-9999
Bae, Hyoung Won(배형원) ORCID logo https://orcid.org/0000-0002-8421-5636
Seong, Gong Je(성공제) ORCID logo https://orcid.org/0000-0002-5456-4296
Lee, Sang Yeop(이상엽) ORCID logo https://orcid.org/0000-0002-3834-7953
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