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Effect of Anti-vascular Endothelial Growth Factor Antibody on the Survival of Cultured Retinal Ganglion Cells
DC Field | Value | Language |
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dc.contributor.author | 김찬윤 | - |
dc.contributor.author | 배형원 | - |
dc.contributor.author | 성공제 | - |
dc.contributor.author | 이상엽 | - |
dc.date.accessioned | 2018-07-20T07:50:20Z | - |
dc.date.available | 2018-07-20T07:50:20Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 1011-8942 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/160573 | - |
dc.description.abstract | PURPOSE: To investigate the effects of anti-vascular endothelial growth factor (VEGF) antibody on the survival of retinal ganglion cell (RGC)-5 cells differentiated with staurosporine under oxidative stress. METHODS: We used real-time polymerase chain reaction and Western blot to confirm the expression of VEGF, VEGF receptor (VEGFR)-1 and VEGFR-2 in RGC-5 cells differentiated with staurosporine for 6 hours. The differentiated RGC-5 cells were treated with 800 μM hydrogen peroxide (H₂O₂) for 24 hours to induce oxidative stress. Then, the survival rate of RGC-5 was confirmed by lactate dehydrogenase assay at each concentration (0, 0.01, 0.1, and 1 mg) using bevacizumab as the anti-VEGF antibody. The expression of VEGF, VEGFR-1, and VEGFR-2 was confirmed using real-time polymerase chain reaction. RESULTS: VEGF, VEGFR-1, and VEGFR-2 were all expressed in differentiated RGC-5 cells. When RGC-5 cells were simultaneously treated with bevacizumab and 800 μM H₂O₂, survival of RGC-5 decreased with bevacizumab concentration. VEGF expression in RGC-5 cells increased with increasing concentration of bevacizumab. Similar patterns were observed for VEGFR-1 and VEGFR-2, but the degree of increase was smaller than that for VEGF. CONCLUSIONS: When bevacizumab was administered to differentiated RGC-5 cells, the cell damage caused by oxidative stress increased. Therefore, given these in vitro study results, caution should be exercised with bevacizumab treatment. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | KJO | - |
dc.relation.isPartOf | Korean Journal of Ophthalmology | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Angiogenesis Inhibitors/pharmacology | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antibodies/pharmacology* | - |
dc.subject.MESH | Bevacizumab/pharmacology | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Cell Differentiation | - |
dc.subject.MESH | Cell Survival | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Gene Expression Regulation/drug effects | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Oxidative Stress/genetics* | - |
dc.subject.MESH | RNA/genetics* | - |
dc.subject.MESH | Real-Time Polymerase Chain Reaction | - |
dc.subject.MESH | Retinal Ganglion Cells/cytology* | - |
dc.subject.MESH | Vascular Endothelial Growth Factor A/biosynthesis | - |
dc.subject.MESH | Vascular Endothelial Growth Factor A/genetics | - |
dc.subject.MESH | Vascular Endothelial Growth Factor A/immunology* | - |
dc.title | Effect of Anti-vascular Endothelial Growth Factor Antibody on the Survival of Cultured Retinal Ganglion Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Ophthalmology | - |
dc.contributor.googleauthor | Ji Min Lee | - |
dc.contributor.googleauthor | Hyoung Won Bae | - |
dc.contributor.googleauthor | Sang Yeop Lee | - |
dc.contributor.googleauthor | Gong Je Seong | - |
dc.contributor.googleauthor | Chan Yun Kim | - |
dc.identifier.doi | 10.3341/kjo.2017.0054 | - |
dc.contributor.localId | A01035 | - |
dc.contributor.localId | A01814 | - |
dc.contributor.localId | A01946 | - |
dc.contributor.localId | A02820 | - |
dc.relation.journalcode | J02931 | - |
dc.identifier.eissn | 2092-9382 | - |
dc.identifier.pmid | 28752700 | - |
dc.subject.keyword | Anti-vascular endothelial growth factor | - |
dc.subject.keyword | Bevacizumab | - |
dc.subject.keyword | Oxidative stress | - |
dc.subject.keyword | RGC-5 | - |
dc.subject.keyword | Retinal ganglion cell | - |
dc.contributor.alternativeName | Kim, Chan Yun | - |
dc.contributor.alternativeName | Bae, Hyoung Won | - |
dc.contributor.alternativeName | Seong, Gong Je | - |
dc.contributor.alternativeName | Lee, Sang Yeop | - |
dc.contributor.affiliatedAuthor | Kim, Chan Yun | - |
dc.contributor.affiliatedAuthor | Bae, Hyoung Won | - |
dc.contributor.affiliatedAuthor | Seong, Gong Je | - |
dc.contributor.affiliatedAuthor | Lee, Sang Yeop | - |
dc.citation.volume | 31 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 360 | - |
dc.citation.endPage | 365 | - |
dc.identifier.bibliographicCitation | Korean Journal of Ophthalmology, Vol.31(4) : 360-365, 2017 | - |
dc.identifier.rimsid | 47654 | - |
dc.type.rims | ART | - |
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