Cited 10 times in
Complementary utility of targeted next-generation sequencing and immunohistochemistry panels as a screening platform to select targeted therapy for advanced gastric cancer
DC Field | Value | Language |
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dc.contributor.author | 금웅섭 | - |
dc.contributor.author | 김현기 | - |
dc.contributor.author | 김형일 | - |
dc.contributor.author | 김효송 | - |
dc.contributor.author | 노성훈 | - |
dc.contributor.author | 라선영 | - |
dc.contributor.author | 박규현 | - |
dc.contributor.author | 박준철 | - |
dc.contributor.author | 배수진 | - |
dc.contributor.author | 백순명 | - |
dc.contributor.author | 범승훈 | - |
dc.contributor.author | 손태일 | - |
dc.contributor.author | 신성관 | - |
dc.contributor.author | 이상길 | - |
dc.contributor.author | 이용찬 | - |
dc.contributor.author | 이은영 | - |
dc.contributor.author | 이한나 | - |
dc.contributor.author | 임준석 | - |
dc.contributor.author | 정민규 | - |
dc.contributor.author | 정재호 | - |
dc.contributor.author | 정현철 | - |
dc.contributor.author | 최윤영 | - |
dc.contributor.author | 형우진 | - |
dc.date.accessioned | 2018-07-20T07:35:44Z | - |
dc.date.available | 2018-07-20T07:35:44Z | - |
dc.date.issued | 2017 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/160321 | - |
dc.description.abstract | We tested the clinical utility of combined profiling of Ion Torrent PGM based next-generation sequencing (NGS) and immunohistochemistry (IHC) for assignment to molecularly targeted therapies. A consecutive cohort of 93 patients with advanced/metastatic GC who underwent palliative chemotherapy between March and December 2015 were prospectively enrolled. Formalin fixed paraffin embedded tumor biopsy specimens were subjected to a 10 GC panels [Epstein Barr virus encoding RNA in-situ hybridization, IHC for mismatch repair proteins (MMR; MLH1, PMS2, MSH2, and MSH6), receptor tyrosine kinases (HER2, EGFR, and MET), PTEN, and p53 protein], and a commercial targeted NGS panel of 52 genes (Oncomine Focus Assay). Treatment was based on availability of targeted agents at the time of molecular diagnosis. Among the 81 cases with available tumor samples, complete NGS and IHC profiles were successfully achieved in 66 cases (81.5%); only IHC results were available for 15 cases. Eight cases received matched therapy based on sequencing results; ERBB2 amplification, trastuzumab (n = 4); PIK3CA mutation, Akt inhibitor (n = 2); and FGFR2 amplification, FGFR2b inhibitor (n = 2). Eleven cases received matched therapy based on IHC; ERBB2 positivity, trastuzumab (n = 5); PTEN loss (n = 2), PI3Kβ inhibitor; MMR deficiency (n = 2), PD-1 inhibitor; and EGFR positivity (n = 2), pan-ERBB inhibitor. A total of 19 (23.5%) and 62 (76.5%) cases were treated with matched and non-matched therapy, respectively. Matched therapy had significantly higher overall response rate than non-matched therapy (55.6% vs 13.1%, P = 0.001). NGS and IHC markers provide complementary utility in identifying patients who may benefit from targeted therapies. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | Impact Journals | - |
dc.relation.isPartOf | ONCOTARGET | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Aged | - |
dc.subject.MESH | Biomarkers | - |
dc.subject.MESH | Tumor/analysis | - |
dc.subject.MESH | Biomarkers | - |
dc.subject.MESH | Tumor/genetics | - |
dc.subject.MESH | Feasibility Studies | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | High-Throughput Nucleotide Sequencing/methods | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunohistochemistry/methods | - |
dc.subject.MESH | Kaplan-Meier Estimate | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Middle Aged | - |
dc.subject.MESH | Molecular Targeted Therapy/methods | - |
dc.subject.MESH | Stomach Neoplasms/drug therapy | - |
dc.subject.MESH | Stomach Neoplasms/genetics | - |
dc.subject.MESH | Stomach Neoplasms/mortality | - |
dc.title | Complementary utility of targeted next-generation sequencing and immunohistochemistry panels as a screening platform to select targeted therapy for advanced gastric cancer | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Radiation Oncology | - |
dc.contributor.googleauthor | Hyo Song Kim | - |
dc.contributor.googleauthor | Hanna Lee | - |
dc.contributor.googleauthor | Su-Jin Shin | - |
dc.contributor.googleauthor | Seung-Hoon Beom | - |
dc.contributor.googleauthor | Minkyu Jung | - |
dc.contributor.googleauthor | Sujin Bae | - |
dc.contributor.googleauthor | Eun Young Lee | - |
dc.contributor.googleauthor | Kyu Hyun Park | - |
dc.contributor.googleauthor | Yoon Young Choi | - |
dc.contributor.googleauthor | Taeil Son | - |
dc.contributor.googleauthor | Hyoung-Il Kim | - |
dc.contributor.googleauthor | Jae-Ho Cheong | - |
dc.contributor.googleauthor | Woo Jin Hyung | - |
dc.contributor.googleauthor | Jun Chul Park | - |
dc.contributor.googleauthor | Sung Kwan Shin | - |
dc.contributor.googleauthor | Sang Kil Lee | - |
dc.contributor.googleauthor | Yong Chan Lee | - |
dc.contributor.googleauthor | Woong Sub Koom | - |
dc.contributor.googleauthor | Joon Seok Lim | - |
dc.contributor.googleauthor | Hyun Cheol Chung | - |
dc.contributor.googleauthor | Sung Hoon Noh | - |
dc.contributor.googleauthor | Sun Young Rha | - |
dc.contributor.googleauthor | Hyunki Kim | - |
dc.contributor.googleauthor | Soonmyung Paik | - |
dc.identifier.doi | 10.18632/oncotarget.16409 | - |
dc.contributor.localId | A00273 | - |
dc.contributor.localId | A01108 | - |
dc.contributor.localId | A01154 | - |
dc.contributor.localId | A01202 | - |
dc.contributor.localId | A01281 | - |
dc.contributor.localId | A01316 | - |
dc.contributor.localId | A04566 | - |
dc.contributor.localId | A01676 | - |
dc.contributor.localId | A05344 | - |
dc.contributor.localId | A01823 | - |
dc.contributor.localId | A04581 | - |
dc.contributor.localId | A01998 | - |
dc.contributor.localId | A02112 | - |
dc.contributor.localId | A02812 | - |
dc.contributor.localId | A02988 | - |
dc.contributor.localId | A03042 | - |
dc.contributor.localId | A03275 | - |
dc.contributor.localId | A03408 | - |
dc.contributor.localId | A03606 | - |
dc.contributor.localId | A03717 | - |
dc.contributor.localId | A03773 | - |
dc.contributor.localId | A04138 | - |
dc.contributor.localId | A04382 | - |
dc.relation.journalcode | J02421 | - |
dc.identifier.eissn | 1949-2553 | - |
dc.identifier.pmid | 28418920 | - |
dc.subject.keyword | gastric cancer | - |
dc.subject.keyword | immunohistochemistry | - |
dc.subject.keyword | matched therapy | - |
dc.subject.keyword | molecular subtypes | - |
dc.subject.keyword | next-generation sequencing | - |
dc.contributor.alternativeName | Koom, Woong Sub | - |
dc.contributor.alternativeName | Kim, Hyun Ki | - |
dc.contributor.alternativeName | Kim, Hyoung Il | - |
dc.contributor.alternativeName | Kim, Hyo Song | - |
dc.contributor.alternativeName | Noh, Sung Hoon | - |
dc.contributor.alternativeName | Rha, Sun Young | - |
dc.contributor.alternativeName | Park, Kyu Hyun | - |
dc.contributor.alternativeName | Park, Jun Chul | - |
dc.contributor.alternativeName | Bae, Sujin | - |
dc.contributor.alternativeName | Paik, Soon Myung | - |
dc.contributor.alternativeName | Beum, Seung Hoon | - |
dc.contributor.alternativeName | Son, Tae Il | - |
dc.contributor.alternativeName | Shin, Sung Kwan | - |
dc.contributor.alternativeName | Lee, Sang Kil | - |
dc.contributor.alternativeName | Lee, Yong Chan | - |
dc.contributor.alternativeName | Lee, Eun Young | - |
dc.contributor.alternativeName | Lee, Hanna | - |
dc.contributor.alternativeName | Lim, Joon Seok | - |
dc.contributor.alternativeName | Jung, Min Kyu | - |
dc.contributor.alternativeName | Cheong, Jae Ho | - |
dc.contributor.alternativeName | Chung, Hyun Cheol | - |
dc.contributor.alternativeName | Choi, Yoon Young | - |
dc.contributor.alternativeName | Hyung, Woo Jin | - |
dc.contributor.affiliatedAuthor | Koom, Woong Sub | - |
dc.contributor.affiliatedAuthor | Kim, Hyun Ki | - |
dc.contributor.affiliatedAuthor | Kim, Hyoung Il | - |
dc.contributor.affiliatedAuthor | Kim, Hyo Song | - |
dc.contributor.affiliatedAuthor | Noh, Sung Hoon | - |
dc.contributor.affiliatedAuthor | Rha, Sun Young | - |
dc.contributor.affiliatedAuthor | Park, Kyu Hyun | - |
dc.contributor.affiliatedAuthor | Park, Jun Chul | - |
dc.contributor.affiliatedAuthor | Bae, Sujin | - |
dc.contributor.affiliatedAuthor | Paik, Soon Myung | - |
dc.contributor.affiliatedAuthor | Beum, Seung Hoon | - |
dc.contributor.affiliatedAuthor | Son, Tae Il | - |
dc.contributor.affiliatedAuthor | Shin, Sung Kwan | - |
dc.contributor.affiliatedAuthor | Lee, Sang Kil | - |
dc.contributor.affiliatedAuthor | Lee, Yong Chan | - |
dc.contributor.affiliatedAuthor | Lee, Eun Young | - |
dc.contributor.affiliatedAuthor | Lee, Hanna | - |
dc.contributor.affiliatedAuthor | Lim, Joon Seok | - |
dc.contributor.affiliatedAuthor | Jung, Min Kyu | - |
dc.contributor.affiliatedAuthor | Cheong, Jae Ho | - |
dc.contributor.affiliatedAuthor | Chung, Hyun Cheol | - |
dc.contributor.affiliatedAuthor | Choi, Yoon Young | - |
dc.contributor.affiliatedAuthor | Hyung, Woo Jin | - |
dc.citation.volume | 8 | - |
dc.citation.number | 24 | - |
dc.citation.startPage | 38389 | - |
dc.identifier.bibliographicCitation | ONCOTARGET , Vol.8(24) : 38389, 2017 | - |
dc.identifier.rimsid | 40982 | - |
dc.type.rims | ART | - |
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