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Elucidation of Gene Expression Patterns in the Brain after Spinal Cord Injury

Authors
 Ahreum Baek  ;  Sung-Rae Cho  ;  Sung Hoon Kim 
Citation
 CELL TRANSPLANTATION, Vol.26(7) : 1286-1300, 2017 
Journal Title
 CELL TRANSPLANTATION 
ISSN
 0963-6897 
Issue Date
2017
MeSH
Animals ; Blotting, Western ; Gene Expression Profiling* ; Gene Expression Regulation* ; Male ; Mice, Inbred ICR ; Models, Biological ; Real-Time Polymerase Chain Reaction ; Reproducibility of Results ; Signal Transduction/genetics ; Spinal Cord Injuries/genetics* ; Transcriptome/genetics
Keywords
brain ; enriched pathways ; spinal cord injury ; transcriptome analysis
Abstract
Spinal cord injury (SCI) is a devastating neurological disease. The pathophysiological mechanisms of SCI have been reported to be relevant to central nervous system injury such as brain injury. In this study, gene expression of the brain after SCI was elucidated using transcriptome analysis to characterize the temporal changes in global gene expression patterns in a SCI mouse model. Subjects were randomly classified into 3 groups: sham control, acute (3 h post-injury), and subacute (2 wk post-injury) groups. We sought to confirm the genes differentially expressed between post-injured groups and sham control group. Therefore, we performed transcriptome analysis to investigate the enriched pathways associated with pathophysiology of the brain after SCI using Database for Annotation Visualization, and Integrated Discovery (DAVID), which yielded Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Following enriched pathways were found in the brain: oxidative phosphorylation pathway; inflammatory response pathways-cytokine-cytokine receptor interaction and chemokine signaling pathway; and endoplasmic reticulum (ER) stress-related pathways-antigen processing and presentation and mitogen-activated protein kinase signaling pathway. Oxidative phosphorylation pathway was identified at acute phase, while inflammation response and ER stress-related pathways were identified at subacute phase. Since the following pathways-oxidative phosphorylation pathway, inflammatory response pathways, and ER stress-related pathways-have been well known in the SCI, we suggested a link between SCI and brain injury. These mechanisms provide valuable reference data for better understanding pathophysiological processes in the brain after SCI.
Files in This Item:
T201702082.pdf Download
DOI
10.1177/0963689717715822
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Rehabilitation Medicine (재활의학교실) > 1. Journal Papers
Yonsei Authors
Cho, Sung-Rae(조성래) ORCID logo https://orcid.org/0000-0003-1429-2684
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/160315
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