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The Combinational Treatment of Antioxidants Protects Brain Endothelial Cells Against Oxygen-Glucose Deprivation Followed by Reperfusion Induced Injury

Authors
 So Mang Kang  ;  Juhyun Song  ;  Won Taek Lee  ;  Kyung Ah Park  ;  Kyoung Min Lee  ;  Jong Eun Lee 
Citation
 Vascular Neurology, Vol.5 : 35-41, 2013 
Journal Title
 Vascular Neurology 
ISSN
 2092-6855 
Issue Date
2013
Keywords
Dehydroascorbic acid ; Glutathione ; Melatonin ; Oxygen-glucose deprivation followed by reperfusion ; Murine brain endothelial cells
Abstract
Antioxidants including dehydroascorbic acid (DHA), glutathione (GSH) and melatonin have cellular protective effects on murine brain endothelial (bEnd.3) cells after oxidative stress and ischemic injury. Up to date, there are no studies that the combinational treatment of these antioxidants boosts the cellular protective effect on bEnd.3 cells. In this study, we investigated the protective effect of antioxidant combinational treatment on bEnd.3 cells after oxygen-glucose deprivation followed by reperfusion (OGD/R) induced injury. The effect of antioxidants combinational treatment on the bEnd.3 cells was examined by cell viability test, western blot analysis, immunocytochemistry. The combinational treatment of DHA (1 mM), GSH (1 mM) protects bEnd.3 cells after OGD/R induced injury and also melatonin (10 μM) treatment boosts the protective effect in combinational treatment of DHA and GSH on bEnd.3 cells after OGD/R induced injury. Western blot analysis suggests that the combinational treatment of DHA, GSH and melatonin attenuated the expression of vascular endothelial growth factor (VEGF), phosphorylation of ERK compared with each single treatment after OGD/R induced injury. Claudin 5, hypoxia inducible factor-1α (HIF-1α) and VEGF immunostaining data suggests the preventive effect of antioxidants combinational treatment. In conclusion, the combinational treatment of antioxidants elevates the cellular protective effect on bEnd.3cells against OGD/R induced injury.
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Park, Kyung Ah(박경아)
Song, Ju Hyun(송주현)
Lee, Won Taek(이원택) ORCID logo https://orcid.org/0000-0001-7348-9562
Lee, Jong Eun(이종은) ORCID logo https://orcid.org/0000-0001-6203-7413
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/158390
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