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House dust mite allergen Der f 1 induces IL-8 in human basophilic cells via ROS-ERK and p38 signal pathways

Authors
 Yi Myung-hee  ;  Kim Hyoung-Pyo  ;  Jeong Kyoung Yong  ;  Kim Chung-Ryul  ;  Kim Tae Yun  ;  Yong Tai-Soon 
Citation
 Cytokine, Vol.75(2) : 356-364, 2015 
Journal Title
 Cytokine 
ISSN
 1043-4666 
Issue Date
2015
MeSH
Acetylcysteine/pharmacology ; Antigens, Dermatophagoides/immunology* ; Arthropod Proteins/immunology* ; Basophils/immunology* ; Cell Line, Tumor ; Cloning, Molecular ; Cysteine Endopeptidases/immunology* ; Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors ; Extracellular Signal-Regulated MAP Kinases/metabolism* ; Humans ; Immunoglobulin E/immunology ; Inflammation/immunology ; Interleukin-8/biosynthesis* ; Interleukin-8/genetics ; Phosphorylation/drug effects ; Phosphorylation/immunology ; RNA, Messenger/biosynthesis ; Reactive Oxygen Species/metabolism* ; Signal Transduction/immunology ; p38 Mitogen-Activated Protein Kinases/antagonists & inhibitors ; p38 Mitogen-Activated Protein Kinases/metabolism*
Keywords
Basophil ; Cysteine protease ; Der f 1 ; IL-8 ; KU812
Abstract
Der f 1, a major house dust mite allergen and member of the papain-like cysteine protease family, can provoke immune responses with its proteolytic activity. To understand the role of Der f 1 in inflammatory immune responses, we studied the mechanism of the regulation of interleukin (IL)-8 expressions in human basophilic cell KU812 by proteolytically active recombinant Der f 1. Not only production of IL-8 mRNA was induced but also the DNA binding activity of activator protein-1 (AP-1) and phosphorylation of NF-κB p65 were increased in Der f 1-treated KU812. Furthermore, Der f 1 induction of IL-8 expression was sensitive to pharmacological inhibition of ERK and p38 mitogen activated protein kinase (MAPK) pathways. Der f 1 also activated ERK and p38 MAPK phosphorylation and rapidly induced reactive oxygen species (ROS) production. The antioxidant N-acetyl-cysteine (NAC) inhibited phosphorylation of ERK, but not p38, suggesting that secretion of IL-8 in KU812 cells treated with Der f 1 is dependent on ROS, ERK MAPK and p38 MAPK. We describe the mechanism of Der f 1-induced IL-8 secretion from human basophilic cells, which are thought to be important for allergic inflammation independent of IgE antibodies. These findings improve our understanding of the inflammatory immune response in human basophils to protease allergens.
Full Text
https://www.sciencedirect.com/science/article/pii/S1043466615300211
DOI
10.1016/j.cyto.2015.07.011
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
Yonsei Authors
김태윤(Kim,​ Tae Yun)
김형표(Kim, Hyoung Pyo) ORCID logo https://orcid.org/0000-0003-1441-8822
용태순(Yong, Tai Soon) ORCID logo https://orcid.org/0000-0002-3445-0769
이명희(Yi, Myung Hee) ORCID logo https://orcid.org/0000-0001-9537-5726
정경용(Jeong, Kyoung Yong) ORCID logo https://orcid.org/0000-0001-9887-1426
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/157198
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