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House dust mite allergen Der f 1 induces IL-8 in human basophilic cells via ROS-ERK and p38 signal pathways

DC Field Value Language
dc.contributor.author김태윤-
dc.contributor.author김형표-
dc.contributor.author용태순-
dc.contributor.author이명희-
dc.contributor.author정경영-
dc.contributor.author정경용-
dc.date.accessioned2018-03-26T17:06:31Z-
dc.date.available2018-03-26T17:06:31Z-
dc.date.issued2015-
dc.identifier.issn1043-4666-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/157198-
dc.description.abstractDer f 1, a major house dust mite allergen and member of the papain-like cysteine protease family, can provoke immune responses with its proteolytic activity. To understand the role of Der f 1 in inflammatory immune responses, we studied the mechanism of the regulation of interleukin (IL)-8 expressions in human basophilic cell KU812 by proteolytically active recombinant Der f 1. Not only production of IL-8 mRNA was induced but also the DNA binding activity of activator protein-1 (AP-1) and phosphorylation of NF-κB p65 were increased in Der f 1-treated KU812. Furthermore, Der f 1 induction of IL-8 expression was sensitive to pharmacological inhibition of ERK and p38 mitogen activated protein kinase (MAPK) pathways. Der f 1 also activated ERK and p38 MAPK phosphorylation and rapidly induced reactive oxygen species (ROS) production. The antioxidant N-acetyl-cysteine (NAC) inhibited phosphorylation of ERK, but not p38, suggesting that secretion of IL-8 in KU812 cells treated with Der f 1 is dependent on ROS, ERK MAPK and p38 MAPK. We describe the mechanism of Der f 1-induced IL-8 secretion from human basophilic cells, which are thought to be important for allergic inflammation independent of IgE antibodies. These findings improve our understanding of the inflammatory immune response in human basophils to protease allergens.-
dc.description.statementOfResponsibilityrestriction-
dc.languageCYTOKINE-
dc.publisherCYTOKINE-
dc.relation.isPartOfCYTOKINE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAcetylcysteine/pharmacology-
dc.subject.MESHAntigens, Dermatophagoides/immunology*-
dc.subject.MESHArthropod Proteins/immunology*-
dc.subject.MESHBasophils/immunology*-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHCloning, Molecular-
dc.subject.MESHCysteine Endopeptidases/immunology*-
dc.subject.MESHExtracellular Signal-Regulated MAP Kinases/antagonists & inhibitors-
dc.subject.MESHExtracellular Signal-Regulated MAP Kinases/metabolism*-
dc.subject.MESHHumans-
dc.subject.MESHImmunoglobulin E/immunology-
dc.subject.MESHInflammation/immunology-
dc.subject.MESHInterleukin-8/biosynthesis*-
dc.subject.MESHInterleukin-8/genetics-
dc.subject.MESHPhosphorylation/drug effects-
dc.subject.MESHPhosphorylation/immunology-
dc.subject.MESHRNA, Messenger/biosynthesis-
dc.subject.MESHReactive Oxygen Species/metabolism*-
dc.subject.MESHSignal Transduction/immunology-
dc.subject.MESHp38 Mitogen-Activated Protein Kinases/antagonists & inhibitors-
dc.subject.MESHp38 Mitogen-Activated Protein Kinases/metabolism*-
dc.titleHouse dust mite allergen Der f 1 induces IL-8 in human basophilic cells via ROS-ERK and p38 signal pathways-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Environmental Medical Biology-
dc.contributor.googleauthorYi Myung-hee-
dc.contributor.googleauthorKim Hyoung-Pyo-
dc.contributor.googleauthorJeong Kyoung Yong-
dc.contributor.googleauthorKim Chung-Ryul-
dc.contributor.googleauthorKim Tae Yun-
dc.contributor.googleauthorYong Tai-Soon-
dc.identifier.doi10.1016/j.cyto.2015.07.011-
dc.contributor.localIdA01077-
dc.contributor.localIdA01163-
dc.contributor.localIdA02424-
dc.contributor.localIdA02761-
dc.contributor.localIdA03572-
dc.relation.journalcodeJ00691-
dc.identifier.eissn1096-0023-
dc.identifier.pmid26194066-
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S1043466615300211-
dc.subject.keywordBasophil-
dc.subject.keywordCysteine protease-
dc.subject.keywordDer f 1-
dc.subject.keywordIL-8-
dc.subject.keywordKU812-
dc.contributor.alternativeNameKim, Tae Yun-
dc.contributor.alternativeNameKim, Hyoung Pyo-
dc.contributor.alternativeNameYong, Tai Soon-
dc.contributor.alternativeNameYi, Myung Hee-
dc.contributor.alternativeNameChung, Kyung Young-
dc.contributor.affiliatedAuthorKim, Tae Yun-
dc.contributor.affiliatedAuthorKim, Hyoung Pyo-
dc.contributor.affiliatedAuthorYong, Tai Soon-
dc.contributor.affiliatedAuthorYi, Myung Hee-
dc.contributor.affiliatedAuthorJeong, Kyoung Yong-
dc.citation.volume75-
dc.citation.number2-
dc.citation.startPage356-
dc.citation.endPage364-
dc.identifier.bibliographicCitationCYTOKINE, Vol.75(2) : 356-364, 2015-
dc.identifier.rimsid41759-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers

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