237 590

Cited 28 times in

Ciliary Phosphoinositide Regulates Ciliary Protein Trafficking in Drosophila

Authors
 Jina Park  ;  Nayoung Lee  ;  Adriana Kavoussi  ;  Jeong Taeg Seo  ;  Chul Hoon Kim  ;  Seok Jun Moon 
Citation
 CELL REPORTS, Vol.13(12) : 2808-2816, 2015 
Journal Title
CELL REPORTS
Issue Date
2015
MeSH
Animals ; Cell Movement/physiology ; Cilia/metabolism* ; Drosophila/metabolism* ; Phosphatidylinositols/metabolism* ; Protein Transport ; Signal Transduction
Abstract
Cilia are highly specialized antennae-like cellular organelles. Inositol polyphosphate 5-phosphatase E (INPP5E) converts PI(4,5)P2 into PI4P and is required for proper ciliary function. Although Inpp5e mutations are associated with ciliopathies in humans and mice, the precise molecular role INPP5E plays in cilia remains unclear. Here, we report that Drosophila INPP5E (dINPP5E) regulates ciliary protein trafficking by controlling the phosphoinositide composition of ciliary membranes. Mutations in dInpp5e lead to hearing deficits due to the mislocalization of dTULP and mechanotransduction channels, Inactive and NOMPC, in chordotonal cilia. Both loss of dINPP5E and ectopic expression of the phosphatidylinositol-4-phosphate 5-kinase Skittles increase PI(4,5)P2 levels in the ciliary base. The fact that Skittles expression phenocopies the dInpp5e mutants confirms a central role for PI(4,5)P2 in the regulation of dTULP, Inactive, and NOMPC localization. These data suggest that the spatial localization and levels of PI(4,5)P2 in ciliary membranes are important regulators of ciliary trafficking and function.
Files in This Item:
T201505046.pdf Download
DOI
10.1016/j.celrep.2015.12.009
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chul Hoon(김철훈) ORCID logo https://orcid.org/0000-0002-7360-429X
Moon, Seok Jun(문석준) ORCID logo https://orcid.org/0000-0001-7282-2888
Park, Ji Na(박지나)
Seo, Jeong Taeg(서정택) ORCID logo https://orcid.org/0000-0003-2697-0251
Lee, Nayoung(이나영)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/157017
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links