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Pannexin 3 is required for normal progression of skeletal development in vertebrates

Authors
 Se-Kyung Oh  ;  Jeong-Oh Shin  ;  Jeong-In Baek  ;  Jinwook Lee  ;  Jae Woong Bae  ;  Harinarayana Ankamerddy  ;  Myoung-Jin Kim  ;  Tae-Lin Huh  ;  Zae-Young Ryoo  ;  Un-Kyung Kim  ;  Jinwoong Bok  ;  Kyu-Yup Lee 
Citation
 FASEB JOURNAL, Vol.29(11) : 4473-4484, 2015 
Journal Title
 FASEB JOURNAL 
ISSN
 0892-6638 
Issue Date
2015
MeSH
Animals ; Calcification, Physiologic/physiology* ; Cell Differentiation/physiology* ; Chondrocytes/cytology ; Chondrocytes/metabolism* ; Connexins/genetics ; Connexins/metabolism* ; Cyclic AMP/genetics ; Cyclic AMP/metabolism ; Mice ; Mice, Knockout ; Osteoblasts/cytology ; Osteoblasts/metabolism* ; Second Messenger Systems/physiology ; Zebrafish/embryology* ; Zebrafish/genetics
Keywords
chondrocytes ; differentiation ; mineralization ; osteoblasts
Abstract
The vertebrate skeletal system has various functions, including support, movement, protection, and the production of blood cells. The development of cartilage and bones, the core components of the skeletal system, is mediated by systematic inter- and intracellular communication among multiple signaling pathways in differentiating progenitors and the surrounding tissues. Recently, Pannexin (Panx) 3 has been shown to play important roles in bone development in vitro by mediating multiple signaling pathways, although its roles in vivo have not been explored. In this study, we generated and analyzed Panx3 knockout mice and examined the skeletal phenotypes of panx3 morphant zebrafish. Panx3(-/-) embryos exhibited delays in hypertrophic chondrocyte differentiation and osteoblast differentiation as well as the initiation of mineralization, resulting in shortened long bones in adulthood. The abnormal progression of hypertrophic chondrogenesis appeared to be associated with the sustained proliferation of chondrocytes, which resulted from increased intracellular cAMP levels. Similarly, osteoblast differentiation and mineralization were delayed in panx3 morphant zebrafish. Taken together, our results provide evidence of the crucial roles of Panx3 in vertebrate skeletal development in vivo.
Full Text
http://www.fasebj.org/content/29/11/4473
DOI
10.1096/fj.15-273722
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Bae, Jae Woong(배재웅)
Bok, Jin Woong(복진웅) ORCID logo https://orcid.org/0000-0003-1958-1872
Shin, Jeong Oh(신정오) ORCID logo https://orcid.org/0000-0002-6935-0936
Ankamreddy, Harinarayana(안캄레디하리) ORCID logo https://orcid.org/0000-0002-2859-087X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/156948
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