55 103

Cited 6 times in

Electrogenic transport and K(+) ion channel expression by the human endolymphatic sac epithelium

Authors
 Sung Huhn Kim  ;  Bo Gyung Kim  ;  Jin Young Kim  ;  Kyung Jin Roh  ;  Michelle J. Suh  ;  Jin Sei Jung  ;  In Seok Moon  ;  Sung K. Moon  ;  Jae Young Choi 
Citation
 SCIENTIFIC REPORTS, Vol.5 : 18110, 2015 
Journal Title
 SCIENTIFIC REPORTS 
Issue Date
2015
MeSH
Chromatography, Liquid ; Cochlea/metabolism ; Cochlea/physiology ; Electrophysiological Phenomena ; Endolymphatic Sac/metabolism ; Endolymphatic Sac/physiology* ; Epithelium/metabolism ; Epithelium/physiology* ; Female ; Gene Expression ; Humans ; Immunohistochemistry ; Ion Transport/physiology ; Male ; Middle Aged ; Potassium/metabolism* ; Potassium Channels/genetics ; Potassium Channels/metabolism ; Potassium Channels/physiology* ; Potassium Channels, Tandem Pore Domain/genetics ; Potassium Channels, Tandem Pore Domain/metabolism ; Potassium Channels, Tandem Pore Domain/physiology ; Reverse Transcriptase Polymerase Chain Reaction ; Small-Conductance Calcium-Activated Potassium Channels/genetics ; Small-Conductance Calcium-Activated Potassium Channels/metabolism ; Small-Conductance Calcium-Activated Potassium Channels/physiology ; Tandem Mass Spectrometry
Abstract
The endolymphatic sac (ES) is a cystic organ that is a part of the inner ear and is connected to the cochlea and vestibule. The ES is thought to be involved in inner ear ion homeostasis and fluid volume regulation for the maintenance of hearing and balance function. Many ion channels, transporters, and exchangers have been identified in the ES luminal epithelium, mainly in animal studies, but there has been no functional study investigating ion transport using human ES tissue. We designed the first functional experiments on electrogenic transport in human ES and investigated the contribution of K(+) channels in the electrogenic transport, which has been rarely identified, even in animal studies, using electrophysiological/pharmacological and molecular biological methods. As a result, we identified functional and molecular evidence for the essential participation of K(+) channels in the electrogenic transport of human ES epithelium. The identified K(+) channels involved in the electrogenic transport were KCNN2, KCNJ14, KCNK2, and KCNK6, and the K(+) transports via those channels are thought to play an important role in the maintenance of the unique ionic milieu of the inner ear fluid.
Files in This Item:
T201504907.pdf Download
DOI
10.1038/srep18110
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sung Huhn(김성헌)
Roh, Kyung Jin(노경진)
Moon, In Seok(문인석) ORCID logo https://orcid.org/0000-0002-3951-5074
Jung, Jinsei(정진세) ORCID logo https://orcid.org/0000-0003-1906-6969
Choi, Jae Young(최재영) ORCID logo https://orcid.org/0000-0001-9493-3458
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/156898
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse