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Establishing quality control ranges for antimicrobial susceptibility testing of Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus: a cornerstone to develop reference strains for Korean clinical microbiology laboratories

 Sung Kuk Hong  ;  Seung Jun Choi  ;  Saeam Shin  ;  Wonmok Lee  ;  Naina Pinto  ;  Nari Shin, Vet  ;  Kwangjun Lee  ;  Seong Geun Hong  ;  Young Ah Kim  ;  Hyukmin Lee  ;  Heejung Kim  ;  Wonkeun Song  ;  Sun Hwa Lee  ;  Dongeun Yong  ;  Kyungwon Lee  ;  Yunsop Chong 
 ANNALS OF LABORATORY MEDICINE, Vol.35(6) : 635-638, 2015 
Journal Title
Issue Date
Anti-Bacterial Agents/pharmacology* ; Asian Continental Ancestry Group ; Escherichia coli/drug effects* ; Humans ; Laboratories ; Microbial Sensitivity Tests/methods* ; Pseudomonas aeruginosa/drug effects* ; Quality Control ; Reference Values ; Republic of Korea ; Staining and Labeling ; Staphylococcus aureus/drug effects*
Agar dilution test ; Antimicrobial susceptibility test ; Clinical microbiology laboratory ; Disk diffusion test ; Quality control ; Reference strain
Quality control (QC) processes are being performed in the majority of clinical microbiology laboratories to ensure the performance of microbial identification and antimicrobial susceptibility testing by using ATCC strains. To obtain these ATCC strains, some inconveniences are encountered concerning the purchase cost of the strains and the shipping time required. This study was focused on constructing a database of reference strains for QC processes using domestic bacterial strains, concentrating primarily on antimicrobial susceptibility testing. Three strains (Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus) that showed legible results in preliminary testing were selected. The minimal inhibitory concentrations (MICs) and zone diameters (ZDs) of eight antimicrobials for each strain were determined according to the CLSI M23. All resulting MIC and ZD ranges included at least 95% of the data. The ZD QC ranges obtained by using the CLSI method were less than 12 mm, and the MIC QC ranges extended no more than five dilutions. This study is a preliminary attempt to construct a bank of Korean QC strains. With further studies, a positive outcome toward cost and time reduction can be anticipated.
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1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Heejung(김희정) ORCID logo https://orcid.org/0000-0002-0190-703X
Shin, Saeam(신새암) ORCID logo https://orcid.org/0000-0003-1501-3923
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Chong, Yun Sop(정윤섭)
Choi, Seung Jun(최승준) ORCID logo https://orcid.org/0000-0003-0736-1055
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