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Hybridization-based aptamer labeling using complementary oligonucleotide platform for PET and optical imaging

Authors
 Jun Young Park  ;  Tae Sup Lee  ;  In Ho Song  ;  Ye Lim Cho  ;  Ju Ri Chae  ;  Mijin Yun  ;  Hyungu Kang  ;  Jung Hwan Lee  ;  Jong Hoon Lim  ;  Won Gil Cho  ;  Won Jun Kang 
Citation
 Biomaterials, Vol.100 : 143-151, 2016 
Journal Title
 Biomaterials 
Issue Date
2016
Abstract
Aptamers are promising next-generation ligands used in molecular imaging and theragnosis. Aptamers are synthetic nucleic acids that can be held together with complementary sequences by base-pair hybridization. In this study, the complementary oligonucleotide (cODN) hybridization-based aptamer conjugation platform was developed to use aptamers as the molecular imaging agent. The cODN was pre-labeled with fluorescent dye or radioisotope and hybridized with a matched sequence containing aptamers in aqueous conditions. The cODN platform-hybridized aptamers exhibited good serum stability and specific binding affinity towards target cancer cells both in vitro and in vivo. These results suggest that the newly designed aptamer conjugation platform offers great potential for the versatile application of aptamers as molecular imaging agents.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X16300213
DOI
10.1016/j.biomaterials.2016.05.035
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Nuclear Medicine (핵의학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Others (기타) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
강원준(Kang, Won Jun) ORCID logo https://orcid.org/0000-0002-2107-8160
박준영(Park, Jun Young)
윤미진(Yun, Mi Jin) ORCID logo https://orcid.org/0000-0002-1712-163X
조예림(Cho, Ye Lim)
채주리(Chae, Ju Ri)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/155734
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