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Ecological changes in oral microcosm biofilm during maturation

Authors
 Young-Seok Kim  ;  Si-Mook Kang  ;  Eun-Song Lee  ;  Ji Hyun Lee  ;  Bo-Ra Kim  ;  Baek-Il Kim 
Citation
 Journal of Biomedical Optics, Vol.21(10) : 101409, 2016 
Journal Title
 Journal of Biomedical Optics 
Issue Date
2016
Abstract
The aim of this study was to evaluate the ecological changes in the biofilm at different stages of maturation using 16S rDNA gene amplicon sequencing and to identify correlations between red/green (R/G) fluorescence ratio and ecological changes. An oral microcosm biofilm was initiated from the saliva of a single donor and grown anaerobically for up to 10 days in basal medium mucin. Quantitative light-induced fluorescence analysis was shown that the R/G ratio of the biofilm increased consistently, but the slope rapidly decreased after six days. The bacterial compositions of 10 species also consistently changed over time. However, there was no significant correlation between each bacteria and red fluorescence. The monitoring of the maturation process of oral microcosm biofilm over 10 days revealed that the R/G ratio and the bacterial composition within biofilm consistently changed. Therefore, the R/G fluorescence ratio of biofilm may be related with its ecological change rather than specific bacteria.
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DOI
10.1117/1.JBO.21.10.101409
Appears in Collections:
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Preventive Dentistry and Public Oral Health (예방치과학교실)
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실)
Yonsei Authors
강시묵(Kang, Si-Mook) ORCID logo https://orcid.org/0000-0003-2341-3636
김백일(Kim, Baek Il) ORCID logo https://orcid.org/0000-0001-8234-2327
김보라(Kim, Bo Ra)
이은송(Lee, Eun Song) ORCID logo https://orcid.org/0000-0002-2949-4783
이지현(Lee, Ji Hyun)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/155730
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