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Identification of cell morphology parameters from automatic hematology analyzers to predict peripheral blood CD34 positive cell count after mobilization

 Saeam Shin  ;  Sung Ran Cho  ;  Sinyoung Kim  ;  Jong Rak Choi  ;  Kyung-A Lee 
 PLoS One, Vol.12(3) : e0174286, 2017 
Journal Title
 PLoS One 
Issue Date
Adult ; Antigens, CD34/blood* ; Area Under Curve ; Blood Component Removal ; Cell Count ; Cell Shape ; Female ; Hematologic Neoplasms/blood* ; Hematologic Neoplasms/pathology ; Hematologic Neoplasms/therapy* ; Hematopoietic Stem Cell Mobilization* ; Hematopoietic Stem Cell Transplantation ; Hematopoietic Stem Cells* ; Humans ; Male ; Middle Aged ; Prospective Studies ; ROC Curve
Optimal timing of apheresis initiation is important for maximizing the hematopoietic stem cell (HSC) yield. This study aimed to identify useful parameters from automatic hematology analyzers for predicting the peripheral blood CD34+ cell count after mobilization. We prospectively enrolled 53 healthy donors and 72 patients, and evaluated 43 cell morphology parameters from Unicel DxH800 (Beckman Coulter, USA) and Advia 2120i (Siemens Healthcare Diagnostics, USA). The correlation of each parameter with the CD34+ cell count in pre-apheresis blood samples was analyzed. The delta neutrophil index (DNI) from Advia 2120i, standard deviation of volume of neutrophils and monocytes (SD-V-NE and SD-V-MO), standard deviation of conductivity of neutrophils and monocytes (SD-C-NE and SD-C-MO), mean conductivity of neutrophils and monocytes (MN-C-NE and MN-C-MO), and standard deviation of axial light loss of neutrophils and monocytes (SD-AL2-NE and SD-AL2-MO) from DxH800 showed significant correlations with the CD34+ cell count. SD-V-NE, SD-C-NE, and SD-C-MO showed good or fair area under the curve values for the prediction of the CD34+ cell count. SD-V-NE, SD-C-NE, and SD-C-MO from DxH800 will provide rapid, useful information for the initiation of apheresis after mobilization.
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1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
김신영(Kim, Sin Young) ORCID logo https://orcid.org/0000-0002-2609-8945
신새암(Shin, Saeam) ORCID logo https://orcid.org/0000-0003-1501-3923
이경아(Lee, Kyung A) ORCID logo https://orcid.org/0000-0001-5320-6705
최종락(Choi, Jong Rak) ORCID logo https://orcid.org/0000-0002-0608-2989
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