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Tubby domain superfamily protein is required for the formation of the 7S SNARE complex in Drosophila

Authors
 Eun Jang Yoon  ;  Yong Taek Jeong  ;  Ji Eun Lee  ;  Seok Jun Moon  ;  Chul Hoon Kim 
Citation
 Biochemical and Biophysical Research Communications, Vol.482(4) : 814-820, 2017 
Journal Title
 Biochemical and Biophysical Research Communications 
ISSN
 0006-291X 
Issue Date
2017
MeSH
Animals ; Animals, Genetically Modified ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism* ; Drosophila melanogaster/genetics ; Drosophila melanogaster/physiology* ; Heat-Shock Response ; Hot Temperature ; Locomotion ; Mutation ; Nerve Net/physiology ; Neurons/physiology ; SNARE Proteins/metabolism* ; Synapses/physiology
Keywords
7S SNARE complex ; Drosophila ; Giant fiber system ; Temperature-sensitive paralysis ; Tubby domain superfamily protein ; Tulp4
Abstract
Tubby domain superfamily protein (TUSP) is a distant member of the Tubby-like protein (TULP) family. Although other TULPs play important roles in sensation, metabolism, and development, the molecular functions of TUSP are completely unknown. Here, we explore the function of TUSP in the Drosophila nervous system where it is expressed in all neurons. Tusp mutant flies exhibit a temperature-sensitive paralysis. This paralysis can be rescued by tissue-specific expression of Tusp in the giant fibers and peripherally synapsing interneurons of the giant fiber system, a well-characterized neuronal circuit that mediates rapid escape behavior in flies. Consistent with this paralytic phenotype, we observed a profound reduction in the assembly of the ternary 7S SNARE complex that is required for neurotransmitter release despite seeing no changes in the expression of each individual SNARE complex component. Together, these data suggest TUSP is a novel regulator of SNARE assembly and, therefore, of neurotransmitter release.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X1631991X
DOI
10.1016/j.bbrc.2016.11.117
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chul Hoon(김철훈) ORCID logo https://orcid.org/0000-0002-7360-429X
Moon, Seok Jun(문석준) ORCID logo https://orcid.org/0000-0001-7282-2888
Jeong, Yong Taek(정용택) ORCID logo https://orcid.org/0000-0003-0067-6396
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/154323
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