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Relative Prevalence and Antimicrobial Susceptibility of Clinical Isolates of Elizabethkingia Species Based on 16S rRNA Gene Sequencing

Authors
 Mi-Soon Han  ;  Hyunsoo Kim  ;  Yangsoon Lee  ;  Myungsook Kim  ;  Nam Su Ku  ;  Jun Yong Choi  ;  Dongeun Yong  ;  Seok Hoon Jeong  ;  Kyungwon Lee  ;  Yunsop Chong 
Citation
 Journal of Clinical Microbiology, Vol.55(1) : 274-280, 2017 
Journal Title
 Journal of Clinical Microbiology 
ISSN
 0095-1137 
Issue Date
2017
MeSH
Aged ; Anti-Bacterial Agents/pharmacology* ; Bacteriological Techniques/methods* ; DNA, Bacterial/chemistry ; DNA, Bacterial/genetics ; DNA, Ribosomal/chemistry ; DNA, Ribosomal/genetics ; Female ; Flavobacteriaceae/classification ; Flavobacteriaceae/drug effects* ; Flavobacteriaceae/genetics ; Flavobacteriaceae/isolation & purification* ; Flavobacteriaceae Infections/epidemiology* ; Flavobacteriaceae Infections/microbiology* ; Genes, rRNA ; Hospitals, University ; Humans ; Male ; Middle Aged ; Prevalence ; RNA, Ribosomal, 16S/genetics ; Republic of Korea/epidemiology ; Sequence Analysis, DNA ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods*
Keywords
16S rRNA gene sequencing ; Elizabethkingia anophelis ; Elizabethkingia meningoseptica ; Elizabethkingia miricola ; antimicrobial susceptibility
Abstract
Some of the previously reported clinical isolates of Elizabethkingia meningoseptica may be later named species of Elizabethkingia We determined the accuracy of species identification (with two matrix-assisted laser desorption ionization-time of flight mass spectrometry [MALDI-TOF MS] systems and the Vitek 2 GN card), relative prevalence of three Elizabethkingia spp. in clinical specimens, and antimicrobial susceptibility of the species identified by 16S rRNA gene sequencing. Specimens for culture were collected from patients in a university hospital in Seoul, South Korea, between 2009 and 2015. All 3 Elizabethkingia spp. were detected in patients; among the 86 isolates identified by 16S rRNA gene sequencing, 17 (19.8%) were E. meningoseptica, 18 (20.9%) were Elizabethkingia miricola, and 51 (59.3%) were Elizabethkingia anophelis Only the MALDI-TOF Vitek MS system with an amended database correctly identified all of the isolates. The majority (76.7%) of the isolates were from the lower respiratory tract, and 8 (9.3%) were from blood. Over 90% of E. meningoseptica and E. anophelis isolates were susceptible to piperacillin-tazobactam and rifampin. In contrast, all E. miricola isolates were susceptible to fluoroquinolones except ciprofloxacin. Further studies are urgently needed to determine the optimal antimicrobial agents for the treatment of infections due to each individual Elizabethkingia species.
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DOI
10.1128/JCM.01637-16
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
구남수(Ku, Nam Su) ORCID logo https://orcid.org/0000-0002-9717-4327
용동은(Yong, Dong Eun) ORCID logo https://orcid.org/0000-0002-1225-8477
이경원(Lee, Kyungwon) ORCID logo https://orcid.org/0000-0003-3788-2134
정석훈(Jeong, Seok Hoon) ORCID logo https://orcid.org/0000-0001-9290-897X
최준용(Choi, Jun Yong) ORCID logo https://orcid.org/0000-0002-2775-3315
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/154130
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